Supplying nitrate before bud break induces pronounced changes in nitrogen nutrition and growth of young poplars
Suraphon Thitithanakul A B C , Gilles Pétel A B , Michel Chalot D E and François Beaujard A B F
+ Author Affiliations
- Author Affiliations
A INRA, UMR547 PIAF, F-63039 Clermont-Ferrand, France.
B Université de Clermont-Ferrand, Université Blaise Pascal, UMR547 PIAF, BP 10448, F-63000 Clermont-Ferrand, France.
C Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Muang Surat Thani 84100, Thailand.
D UMR CNRS 6249 Université de Franche-Comté, Place Leclerc, 25030 Besançon, France.
E Université de Lorraine, Faculté des Sciences and Technologies, 54506 Vandoeuvre-les-Nancy cedex, France.
F Corresponding author. Email: francois.beaujard@clermont.inra.fr
Functional Plant Biology 39(9) 795-803 https://doi.org/10.1071/FP12129
Submitted: 25 April 2012 Accepted: 12 July 2012 Published: 15 August 2012
Abstract
Tree nutrient research concentrated on endogenous C and N remobilisation in spring has neglected to acknowledge the possibilities of significant effects of N uptake before bud break, especially on the quality of regrowth and N reserve remobilisation. To investigate this subject, experimental studies were performed on young poplars (Populus tremula × Populus alba, clone INRA 717–1B4) grown with a controlled nutrient supply: (i) without N, ‘control’; (ii) N supplied throughout the course of the experiment, ‘N-supply’; and (iii) N supplied only before bud break, ‘N-pulse’. Results confirm the hypothesis that poplar scions can significantly take up nitrate before bud break, amounting to ~34% of the total N stored the previous year. After bud break, emerging leaves restart the sap flow, which increased nitrate uptake to support the regrowth. N-pulse and N-supply treatments were found to have significant effects shortly after a growth period, i.e. by increasing N content of all tissues (e.g. 37 and 81% in new shoots respectively), leaf area (18 and 29%) and specific leaf area (20 and 35%). Therefore, results confirm the hypothesis that early N supply plays a significant role in the N status and N remobilisation involved in the spring regrowth of young trees.
Additional keywords: absorption, nitrogen, Poplar, regrowth, remobilisation, spring.
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