Reflectance continuum removal spectral index tracking the xanthophyll cycle photoprotective reactions in Norway spruce needles
Daniel Kováč A F , Martin Navrátil B C , Zbyněk Malenovský D E , Michal Štroch A B , Vladimír Špunda A B and Otmar Urban A
+ Author Affiliations
- Author Affiliations
A Global Change Research Centre AS CR, v.v.i., Bělidla 4a, CZ-60300 Brno, Czech Republic.
B Department of Physics, Faculty of Science, University of Ostrava, Chittussiho 10, CZ-71000 Slezská Ostrava, Czech Republic.
C Karlsruhe Institute of Technology, Botanik II, Kaiserstrasse 12, D-76128 Karlsruhe, Germany.
D Remote Sensing Laboratories, Department of Geography, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.
E School of Geography and Environmental Studies, University of Tasmania, Private Bag 76, Hobart, Tas. 7001, Australia.
F Corresponding author. Email: kovac.d@czechglobe.cz
Functional Plant Biology 39(12) 987-998 https://doi.org/10.1071/FP12107
Submitted: 6 April 2012 Accepted: 24 August 2012 Published: 1 October 2012
Abstract
This laboratory experiment tested the ability of the spectral index called ‘area under curve normalised to maximal band depth’ (ANMB) to track dynamic changes in the xanthophyll cycle of Norway spruce (Picea abies (L.) Karsten) needles. Four-year-old spruce seedlings were gradually acclimated to different photosynthetic photon flux densities (PPFDs) and air temperature regimes. The measurements were conducted at the end of each acclimation period lasting for 11 days. A significant decline in the chlorophylls to carotenoids ratio and the increase of the amount of xanthophyll cycle pigments indicated a higher need for carotenoid-mediated photoprotection in spruce leaves acclimated to high PPFD conditions. Similarly, the photochemical reflectance index (PRI) changed from positive to negative values after changing light conditions from low to high intensity as a consequence of the increase in carotenoid content. Systematic responses of PRI to the de-epoxidation state of xanthophyll cycle pigments (DEPS) were, however, observed only during high temperature treatments and after the exposition of needles to high irradiance. The ANMB index computed from needle reflectance between 507 and 556 nm was able to track dynamic changes in DEPS without any influence induced by changing the content of leaf photosynthetic pigments (chlorophylls, carotenoids).
Additional keywords: diurnal course, growth chambers, photosynthetic pigments composition, Picea abies, reflectance continuum removal, xanthophyll cycle.
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