The role of SORBITOL DEHYDROGENASE in Arabidopsis thaliana
Marta Nosarzewski A C , A. Bruce Downie A , Benhong Wu A B and Douglas D. Archbold A
+ Author Affiliations
- Author Affiliations
A University of Kentucky, Department of Horticulture, N318 Agricultural Science Center North, Lexington, KY 40546, USA.
B Present address: Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
C Corresponding author. Email: mnosarze@uky.edu
Functional Plant Biology 39(6) 462-470 https://doi.org/10.1071/FP12008
Submitted: 12 January 2012 Accepted: 4 April 2012 Published: 17 May 2012
Abstract
SORBITOL DEHYDROGENASE (SDH, EC 1.1.1.14) catalyses the interconversion of polyols and ketoses (e.g. sorbitol ↔ fructose). Using two independent Arabidopsis thaliana (L.) Heynh. sdh knockout mutants, we show that SDH (At5g51970) plays a primary role in sorbitol metabolism as well as an unexpected role in ribitol metabolism. Sorbitol content increased in both wild-type (WT) and mutant plant leaves during drought stress, but mutants showed a dramatically different phenotype, dying even if rewatered. The lack of functional SDH in mutant plants was accompanied by accumulation of foliar sorbitol and at least 10-fold more ribitol, neither of which decreased in mutant plants after rewatering. In addition, mutant plants were uniquely sensitive to ribitol in a concentration-dependent manner, which either prevented them from completing seed germination or inhibited seedling development, effects not observed with other polyols or with ribitol-treated WT plants. Ribitol catabolism may occur solely through SDH in A. thaliana, though at only 30% the rate of that for sorbitol. The results indicate a role for SDH in metabolism of sorbitol to fructose and in ribitol conversion to ribulose in A. thaliana during recovery from drought stress.
Additional keywords: carbohydrate metabolism, polyol metabolism, seed germination.
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