Ageing in embryos from wheat grains stored at different temperatures: oxidative stress and antioxidant response
Carmelina Spanò A B , Stefania Bottega A , Roberto Lorenzi A and Isa Grilli A
+ Author Affiliations
- Author Affiliations
A Dipartimento di Biologia, Università di Pisa, Via L. Ghini 5, 56126 Pisa, Italy.
B Corresponding author. Email: cspano@biologia.unipi.it
Functional Plant Biology 38(7) 624-631 https://doi.org/10.1071/FP11046
Submitted: 14 February 2011 Accepted: 17 May 2011 Published: 12 July 2011
Abstract
In the present work we studied oxidative stress as an important cause of seed deterioration during ageing in embryos from durum wheat grains stored at room temperature and at low temperature (10°C). The protective role of low temperature on seed viability was confirmed. The increase of hydrogen peroxide content during dry storage was strongly correlated with the decrease of germinability. Ascorbate and glutathione showed a good correlation with grain germinability and significantly increased upon imbibition, in particular in embryos from viable grains. Ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione peroxidase (GPX) and catalase (CAT) were studied quantitatively (enzymatic assays). APX, GR, and GPX were also studied qualitatively by native PAGE. The enzymes were active in dry, still viable, embryos whereas no activity was detected in non-viable embryos. With the exception of APX, all enzymatic activities decreased upon imbibition. The study of grains stored in different conditions indicated a negative correlation between the efficiency of the antioxidant enzymatic machinery and the age of the grain. The differences detected in differently stored materials confirmed that both germination parameters and the length of storage period are important in determining grain condition.
Additional keywords: aging, antioxidant molecules, antioxidant response, ROS, storage conditions, Triticum turgidum ssp. durum.
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