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Contents Vol 37(7)

Dynamic quantitative trait loci for salt stress components on chromosome 1 of rice

Tanveer Ul Haq A , John Gorham B , Javaid Akhtar C , Nasim Akhtar D and Katherine A. Steele B E
+ Author Affiliations
- Author Affiliations

A University College of Agriculture, PO Box 79, Dera Ghazi Khan 32200, Pakistan.

B Centre for Advanced Research in International Agricultural Development, College of Natural Sciences, Bangor University, Deiniol Road, Bangor, Gwynedd, Wales LL57 2UW, UK.

C Centre for Advanced Studies in Applied Genetics and Saline Agriculture (CAGSA), University of Agriculture, Faisalabad 38000, Pakistan.

D School of Biological Sciences, College of Natural Sciences, Bangor University, Deiniol Road, Bangor, Gwynedd, Wales LL57 2UW, UK.

E Corresponding author. Email: k.a.steele@bangor.ac.uk

Functional Plant Biology 37(7) 634-645 https://doi.org/10.1071/FP09247
Submitted: 10 October 2009  Accepted: 8 March 2010   Published: 2 July 2010

Abstract

Rice varieties Co39 and Moroberekan differ for leaf Na+ concentrations when grown at moderate salinity (100–150 mol m–3 NaCl; 10 : 1 or 20 : 1 Na+ to Ca2+ ratio). Recombinant inbred lines (RILs) from a cross between them were used to map quantitative trait loci (QTL) under salt stress over several weeks. Two experiments (conducted with 170 and 96 RILs, and a linkage map of 126 RFLP markers) identified a major effect on QTL for leaf Na+ concentration and K+ : Na+ ratio on chromosome 1 in a region corresponding to 11.07–14.6 Mbp. No leaf Cl– QTL were detected. In a third experiment, leaves and sheaths were harvested after 7 and 21 days at 100 mol m–3 NaCl. The linkage map of chromosome 1 was improved by the addition of 28 microsatellite markers, which resolved distinct QTL for Na+ and K+ concentrations, and K+ : Na+ ratio. After 7 days’ stress, the most significant QTL were in the region of 11.56–12.66 Mbp. The highest Na+ concentrations were recorded in the sheaths. Na+ concentration QTL were detected for leaves, but not for sheaths. After 21 days’ stress, the region containing the most significant QTL extended to 11.07 Mbp in leaves and in sheaths. A QTL for the ratio of leaf Na+ to sheath Na+ concentrations was found at 11.39–12.39 Mbp. These findings suggest that multiple genes in this region are involved in the response to salinity, and their impact is dynamic according to stress duration, and leaf age and type.

Additional keywords: ion accumulation, Nax1, Nax2, physiological response, salinity, qSaltol, SalT, SKC1.


Acknowledgements

This work was supported by a split doctoral fellowship to T. Ul Haq under the project ‘Upgrading Human Resources for the Agricultural University of Faisalabad – a sustainable partnership between Pakistan, Germany and the UK’ funded by the Asia Link Program of the European Commission. The contents are the sole responsibility of the authors and can in no way be taken to reflect the views of the European Union. Financial support for the completion of the work from Higher Education Commission (HEC) of Pakistan is thankfully acknowledged. Financial support of the Government of Pakistan in the form of a scholarship to N. Akhtar is also acknowledged. RILs were supplied by Dr Adam Price (University of Aberdeen) and Dr Brigitte Courtois (CIRAD, Montpellier, France), who also supplied the RFLP data used herein.


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