Isolation, phylogeny and evolutionary analysis of Pto-type disease resistance gene analogues from a Cucumis hystrix introgression line of cucumber (C. sativus)
Hongjian Wan A B , Chuntao Qian A , Ahmed Abbas Malik A , Zhenguo Zhao A and Jinfeng Chen A CA State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, People’s Republic of China.
B Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, People’s Republic of China.
C Corresponding author. Email: jfchen@njau.edu.cn
Functional Plant Biology 37(6) 513-523 https://doi.org/10.1071/FP09205
Submitted: 2 August 2009 Accepted: 27 February 2010 Published: 20 May 2010
Abstract
Multiple resistances to bacterial and fungal pathogens due to Pto-type serine/threonine kinase (STK) has made this protein a promising candidate for resistance breeding and provided motivation to identify functional Pto-type genes from other plant species. In our present paper, eight classes of STKs were isolated from the cucumber (Cucumis sativus L.) introgressed line 5211S that shows downy mildew resistance. Phylogenetic analysis revealed six classes of cucumber Pto-type resistance gene analogues (RGAs). Multiple sequence alignment of deduced amino acid sequences from these Pto-type RGAs and Pto showed the typical structure of a Pto protein. Non-synonymous to synonymous nucleotide substitution (Ka : Ks) ratios for these Pto-type RGAs were less than 1, indicating that purifying selection played an important role in the evolutionary process. The Pto-type RGAs identified in this study will permit further genetic characterisation that may lead to the development of specific or even broad-spectrum pathogen resistance in 5211S.
Additional keywords: non-synonymous nucleotide substitution, Pto-RGA, synonymous nucleotide substitution.
Acknowledgements
This research was partially supported by the Key Program (30830079), the General Programs 30671419 and 30700541 from the National Natural Science Foundation of China, and the ‘863’ Programs 2008AA10Z150, 2006AA10Z1A8 and 2006AA100108.
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