Register      Login
Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
Shopping Cart: ( empty )
You are here: Home > Journals > FP > FP09175
RESEARCH FRONT
Contents Vol 37(2)

Partial rootzone drying and deficit irrigation increase stomatal sensitivity to vapour pressure deficit in anisohydric grapevines

Marisa J. Collins A C D , Sigfredo Fuentes B and Edward W. R. Barlow A
+ Author Affiliations
- Author Affiliations

A Melbourne School of Land and Environment, University of Melbourne, Vic. 3010, Australia.

B Centre for Plant and Food Science, University of Western Sydney, Hawkesbury Campus, Richmond, NSW 2753, Australia.

C Present address: CSIRO Plant Industry, PO Box 350, Glen Osmond, SA 5064, Australia.

D Corresponding author. Email: marisa.collins@csiro.au

Functional Plant Biology 37(2) 128-138 https://doi.org/10.1071/FP09175
Submitted: 13 July 2009  Accepted: 22 October 2009   Published: 3 February 2010

Abstract

The aim of this study was to investigate how alternative irrigation strategies affected grapevine (Vitis vinifera L.) stomatal response to atmospheric vapour pressure deficit (VPD). In two sites, application of partial rootzone drying (PRD) at 90–100% of crop evapotranspiration (ETc) increased stomatal sensitivity of Shiraz (Syrah) grapevines to high VPD compared with control vines irrigated with the same amount of water but applied on both sides of the vine. PRD significantly reduced vine water use (ESF) measured as sap flow and in dry conditions increased the depth of water uptake from the soil profile. In both experiments, PRD reduced vine water use by up to 50% at moderate VPD (~3 kPa) compared with control vines irrigated at the same level. In the same vines, the response to PRD applied at 100% ETc and deficit irrigation applied at 65% ETc was the same, increasing stomatal sensitivity to VPD and decreasing sap flow. Hydraulic signalling apparently did not play a role in changing stomatal sensitivity as there was no difference in stem water potentials between any of the treatment (PRD and DI) and control vines. This suggests that a long distance root-based chemical signal such as ABA may be responsible for the changes in stomatal behaviour. Shiraz grapevines have previously been classified as anisohydric-like, but application of PRD and DI increased stomatal closure in response to conditions of high evaporative demand making the vines behave in a more isohydric-like manner.

Additional keywords: root development, stomatal behaviour, Vitis vinifera, water use efficiency.


Acknowledgements

We thank Sonja Needs, Georgina Kelley and Ruben Mora for valuable assistance in making field measurements; Jann Conroy and Gordon Rogers for expert advice in the experimental design; and Brian Loveys for critical comments on the manuscript. A grant from the Australian Grape and Wine Research and Development Corporation supported this work.


References


Antolín MC, Santesteban H, Santa María E, Aguirreolea J, Sánchez-Díaz M (2008) Involvement of abscisic acid and polyamines in berry ripening of Vitis vinifera (L.) subjected to water deficit irrigation. Australian Journal of Grape and Wine Research 14, 123–133.
| Crossref | GoogleScholarGoogle Scholar | [Verified 26 November 2009]

Buckley TN (2005) The control of stomata by water balance. New Phytologist 168, 275–292.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Bunce JA (1985) Effect of boundary layer conductance on the response of stomata to humidity. Plant, Cell & Environment 8, 55–57.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Collins MJ (2006) Physiological responses of field grown Shiraz grapevines to partial rootzone drying and deficit irrigation. PhD Thesis, University of Melbourne.

Davies WJ, Wilkinson S, Loveys B (2002) Stomatal control by chemical signalling and the exploitation of this mechanism to increase water use efficiency in agriculture. New Phytologist 153, 449–460.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

de Souza CR, Maroco JP, dos Santos TP, Rodrigues ML, Lopes CM, Pereira JS, Chaves MM (2003) Partial rootzone drying: regulation of stomatal aperture and carbon assimilation in field-grown grapevines (Vitis vinifera cv. Moscatel). Functional Plant Biology 30, 653–662.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

de Souza CR, Maroco JP, dos Santos TP, Rodrigues ML, Lopes C, Pereira JS, Chaves MM (2005a) Control of stomatal aperture and carbon uptake by deficit irrigation in two grapevine cultivars. Agriculture Ecosystems & Environment 106, 261–274.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

de Souza CR, Maroco JP, dos Santos TP, Rodrigues ML, Lopes CM, Pereira JS, Chaves MM (2005b) Impact of deficit irrigation on water use efficiency and carbon isotope composition (delta 13C) of field-grown grapevines under Mediterranean climate. Journal of Experimental Botany 56, 2163–2172.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Dodd IC (2009) Rhizosphere manipulations to maximize ‘crop per drop’ during deficit irrigation. Journal of Experimental Botany 60, 2454–2459.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Dodd IC, Theobald JC, Bacon MA, Davies WJ (2006) Alternation of wet and dry sides during partial rootzone drying irrigation alters root-to-shoot signalling of abscisic acid. Functional Plant Biology 33, 1081–1089.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Dodd IC, Egea G, Davies WJ (2008) Abscisic acid signalling when soil moisture is heterogeneous: decreased photoperiod sap flow from drying roots limits abscisic acid export to the shoots. Plant, Cell & Environment 31, 1263–1274.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

dos Santos TP, Lopes CM, Rodrigues ML, de Souza CR, Maroco JP, Pereira JS, Silva JR, Chaves MM (2003) Partial rootzone drying: effects on growth and fruit quality of field-grown grapevines (Vitis vinifera). Functional Plant Biology 30, 663–671.
| Crossref | GoogleScholarGoogle Scholar | open url image1

dos Santos TP, Lopes CM, Rodrigues ML, Souza CR, Ricardo-da-Silva JM, Maroco JP, Pereira JS, Chaves MM (2005) Effects of partial root-zone drying irrigation on cluster microclimate and fruit composition of field-grown Castelao grapevines. Vitis 44, 117–125. open url image1

dos Santos TP, Lopes CM, Rodrigues ML, Souza CR, Ricardo-da-Silva JM, Maroco JP, Pereira JS, Chaves MM (2007) Effects of deficit irrigation strategies on cluster microclimate for improving fruit composition of Moscatel field-grown grapevines. Scientia Horticulturae 112, 321–330.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Dry PR, Loveys BR (1999) Grapevine shoot growth and stomatal conductance are reduced when part of the root system is dried. Vitis 38, 151–156. open url image1

Dry PR, Loveys BR, Düring H (2000) Partial drying of the rootzone of grape. II. Changes in the pattern of root development. Vitis 39, 9–12. open url image1

Eamus D, Taylor DT, Macinnis-Ng CMO, Shanahan S, De Silva L (2008) Comparing model predictions and experimental data for the response of stomatal conductance and guard cell turgor to manipulations of cuticular conductance, leaf-to-air vapour pressure difference and temperature: feedback mechanisms are able to account for all observations. Plant, Cell & Environment 31, 269–277.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Edwards WRN, Warrick NWM (1984) Transpiration form a kiwifruit vine as estimated by the heat-pulse technique and the Penman-Monteith equation. New Zealand Journal of Agricultural Research 27, 537–543. open url image1

Ginestar C, Eastham J, Gray S, Iland P (1998) Use of sap-flow sensors to schedule vineyard irrigation. I. Effects of post-veraison water deficits on water relations, vine growth, and yield of Shiraz grapevines. American Journal of Enology and Viticulture 49, 413–420. open url image1

Gong D, Kang S, Zhang J (2005) Responses of canopy transpiration and canopy conductance of peach (Prunus persica) trees to alternate partial root zone drip irrigation. Hydrological Processes 19, 2575–2590.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Green SR, Clothier BE (1988) Water use of kiwifruit vines and apple trees by the heat-pulse technique. Journal of Experimental Botany 39, 115–123.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Green S, Clothier B, Jardine B (2003) Theory and practical application of heat pulse to measure sap flow. Agronomy Journal 95, 1371–1379. open url image1

Gu SL, Du GQ, Zoldoske D, Hakim A, Cochran C, Fugelsang K, Jorgensen G (2004) Effects of irrigation amount on water relations, vegetative growth, yield and fruit composition of Sauvignon Blanc grapevines under partial rootzone drying and conventional irrigation in the San Joaquin Valley of California, USA. The Journal of Horticultural Science & Biotechnology 79, 26–33. open url image1

Heilman JL, McInnes KJ, Gesch RW, Lascano RJ, Savage MJ (1996) Effects of trellising on the energy balance of a vineyard. Agricultural and Forest Meteorology 81, 79–93.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Hennessy K , Fitzharris B , Bates BC , Harvey N , Howden SM , Hughes L , Salinger J , Warrick R (2007) ‘Australia and New Zealand. Climate change 2007: impacts, adaptation and vulnerability.’ (Cambridge University Press: Cambridge, UK)

Intrigliolo DS, Castel JR (2009) Response of Vitis vinifera cv. ‘Tempranillo’ to partial rootzone drying in the field: water relations, growth, yield and fruit and wine quality. Agricultural Water Management 96, 282–292.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Kang S, Hu X, Jerie P, Zhang J (2003) The effects of partial rootzone drying on root, trunk sap flow and water balance in an irrigated pear (Pyrus communis L.) orchard. Journal of Hydrology (Amsterdam) 280, 192–206.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Loveys BR (1991) How useful is a knowledge of ABA physiology for crop improvement? In ‘Abscisic acid: physiology and biochemistry’. (Eds WJ Davies, HG Jones) pp. 245–260. (Bios Scientific Publishing: Oxford)

Loveys BR, Dry PR, Stoll M, McCarthy MG (2000) Using plant physiology to improve the water use efficiency of horticultural crops. Acta Horticulturae 537, 187–197. open url image1

Loveys B , Stoll M , Davies WJ (2004) Physiological approaches to enhance water-use efficiency in agriculture: exploiting plant signalling in novel irrigation practice. In ‘Water use efficiency in plant biology’. (Ed. MA Bacon) pp. 113–141. (Blackwell Publishing: Oxford)

Marshall DC (1958) Measurement of sap flow in conifers by heat transport. Plant Physiology 33, 385–396.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

McCarthy MG , Loveys BR , Dry PR , Stoll M (2002) ‘Regulated deficit irrigation and partial rootzone drying as irrigation management techniques for grapevines.’ (FAO: Rome)

Monteith JL (1995) A reinterpretation of stomatal responses to humidity. Plant, Cell & Environment 18, 357–364.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Mott KA, Parkhurst DF (1991) Stomatal responses to humidity in air and helox. Plant, Cell & Environment 14, 509–515.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Olivo N, Girona J, Marsal J (2009) Seasonal sensitivity of stem water potential to vapour pressure deficit in grapevine. Irrigation Science 27, 175–182.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Poni S, Bernizzoni F, Reinotti M (2005) Whole-canopy and single-leaf gas-exchange responses to partial rootzone drying in potted ‘Cabernet Sauvignon’ grapevines. Acta Horticulturae 689, 277–283. open url image1

Poni S, Bernizzoni F, Civardi S (2007) Response of ‘Sangiovese’ grapevines to partial root-zone drying: gas-exchange, growth and grape composition. Scientia Horticulturae 114, 96–103.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Pudney S , Nicholas PR , Skewes M (2004) Irrigation management. In ‘Soil, irrigation and nutrition’. (Ed. P Nicholas) pp. 148–164. (Winetitles: Adelaide)

Rodrigues ML, Santos TP, Rodrigues AP, de Souza CR, Lopes CM, Maroco JP, Pereira JS, Chaves MM (2008) Hydraulic and chemical signalling in the regulation of stomatal conductance and plant water use in field grapevines growing under deficit irrigation. Functional Plant Biology 35, 565–579.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Sadras VO (2009) Does partial root-zone drying improve irrigation water productivity in the field? A meta-analysis. Irrigation Science 27, 183–190.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Schultz HR (2003) Differences in hydraulic architecture account for near-isohydric and anisohydric behaviour of two field-grown Vitis vinifera L. cultivars during drought. Plant, Cell & Environment 26, 1393–1405.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Sharp RE , Davies WJ (1989) Regulation of growth and development of plants growing with a restricted supply of water. In ‘Plants under stress. Society for Experimental Biology seminar Series’. pp. 71–93. (Cambridge University Press: Cambridge)

Soar CJ, Speirs J, Maffei SM, Penrose AB, McCarthy MG, Loveys BR (2006) Grape vine varieties Shiraz and Grenache differ in their stomatal response to VPD: apparent links with ABA physiology and gene expression in leaf tissue. Australian Journal of Grape and Wine Research 12, 2–12.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Steudle E (2000) Water uptake by roots: effects of water deficit. Journal of Experimental Botany 51, 1531–1542.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Stoll M, Dry P, Loveys B, Stewart D, McCarthy M (2000a) Partial rootzone drying. Effects on root distribution and commercial application of a new irrigation technique. Australian & New Zealand Wine Industry Journal 15, 74–76. open url image1

Stoll M, Loveys B, Dry P (2000b) Hormonal changes induced by partial rootzone drying of irrigated grapevine. Journal of Experimental Botany 51, 1627–1634.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Swanson RH, Whitfield DWA (1981) A numerical analysis of heat-pulse velocity theory and practice. Journal of Experimental Botany 32, 221–239.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Tardieu F, Davies WJ (1992) Stomatal response to abscisic acid is a function of current plant water status. Plant Physiology 98, 540–545.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Tardieu F, Simonneau T (1998) Variability among species of stomatal control under fluctuating soil water status and evaporative demand: modelling isohydric and anisohydric behaviors. Journal of Experimental Botany 49, 419–432.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Vandeleur RK, Mayo G, Shelden MC, Gilliham M, Kaiser BN, Tyerman SD (2009) The role of plasma membrane intrinsic protein aquaporins in water transport through roots: diurnal and drought stress responses reveal different strategies between isohydric and anisohydric cultivars of grapevine. Plant Physiology 149, 445–460.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Wample RL , Smithyman RP (2002) ‘Regulated deficit irrigation as a water management strategy in Vitis vinifera production.’ (FAO: Rome)

Wilkinson S, Davies WJ (2002) ABA-based chemical signalling: the co-ordination of responses to stress in plants. Plant, Cell & Environment 25, 195–210.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Williams LE, Araujo FJ (2002) Correlations among predawn leaf, midday leaf, and midday stem water potential and their correlations with other measures of soil and plant water status in Vitis vinifera. Journal of the American Society for Horticultural Science 127, 448–454. open url image1

Xie XD, Wang YB, Williamson L, Holroyd GH, Tagliavia C , et al. (2006) The identification of genes involved in the stomatal response to reduced atmospheric relative humidity. Current Biology 16, 882–887.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1