Proteomic analysis of ‘hybrid necrosis’ in wheat (Triticum aestivum) leaves
Xing Lai Pan A E , Qi Yan Jiang B D , Qian Ying Pan A C , Xue Fei Wen C , Yin Hong Shi A , Yong Jie Wang A , Tian Yuan Pan A , San Gang Xie A , Gui Yun Zhang A , Shen Jie Wu A , Yong Feng Chai A , Chang Sheng Zhang A , Zong Xin Wu A and Shi Hua Shen B EA Food Crop Science Department, Cotton Research Institute, Shanxi Agriculture Science Academy, Yuncheng, Shanxi 044000, PR China.
B Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, PR China.
C Foreign Language College, Anhui University of Technology and Science, Wuhu, Anhui 241000, PR China.
D Graduate College of the Chinese Academy of Sciences, Beijing 100093, PR China.
E Corresponding author. Email: pxlwbig@126.com
Functional Plant Biology 36(3) 251-259 https://doi.org/10.1071/FP08281
Submitted: 31 October 2008 Accepted: 11 December 2008 Published: 2 March 2009
Abstract
Wheat hybrid necrosis has been genetically characterised for many years, but the specific gene(s) and the protein products involved in the processes remains unknown. In this study, protein expression in the base (B), mid (M) and tip (T) segments of the FL-2 leaves of a necrotic hybrid, PZF1 and its parents, Pan555 and Zheng891, was analysed and compared using a high throughput proteomic approach. Twenty-three protein spots, with significant variations in intensity across the necrotic leaf segments, were analysed by MALDI-TOF-MS, of which, 18 were matched to protein accessions in the NCBI database. Several of these proteins are enzymes involved in the methylation cycle, including AdoHcy hydrolase, AdoMet synthase 3 and methionine synthase 1; AdoHcy hydrolase was downregulated sharply in M and T, and AdoMet synthase 3 and methionine synthase 1 were upregulated gradually from M to T. This result suggests that methylation-associated processes, including epigenetic mechanisms, may play a role in the initiation and development of hybrid necrosis. Several energy cycle-associated proteins and cytoprotective proteins were also differentially expressed across the leaf segments, suggesting their direct association with or possible involvement in the necrotic processes. The significant imbalance of a heat-shock protein, a transposon protein and a RNA- and ssDNA-binding protein also makes these proteins potential molecular components in the necrotic processes.
Additional keywords: breeding, cell death, epigenetics, methylation enzymes, miRNA.
Acknowledgements
This work was supported by the National Natural Science Foundation of China (30571161).
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