A PRp27 gene of Nicotiana benthamiana contributes to resistance to Pseudomonas syringae pv. tabaci but not to Colletotrichum destructivum or Colletotrichum orbiculare
Weilong Xie A and Paul H. Goodwin A BA Department of Environmental Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
B Corresponding author. Email: pgoodwin@uoguelph.ca
Functional Plant Biology 36(4) 351-361 https://doi.org/10.1071/FP08241
Submitted: 12 September 2008 Accepted: 21 January 2009 Published: 1 April 2009
Abstract
NbPRp27 from Nicotiana benthamiana Domin. is highly similar to NtPRp27, which is a secreted protein from Nicotiana tabacum L. belonging to pathogen-inducible genes comprising the PR17 family of pathogenesis-related proteins. A collection of related genes from plants in several plant families showed that their deduced amino acid sequences clustered according to plant family. Expression of NbPRp27 was not detectable in healthy leaves or stems but was expressed at high levels in roots. Expression was induced by wounding, BTH, ethylene, methyl jasmonate, ABA and NAA, but not by drought, heat or cold stress. Expression was induced by infection with the hemibiotrophic pathogens, Colletotrichum destructivum, Colletotrichum orbiculare and Pseudomonas syringae pv. tabaci. For infections with the Colletotrichum species, expression increased more slowly during biotrophy than necrotrophy, but the reverse was true for P. syringae pv. tabaci. Virus-induced silencing of NbPRp27 did not affect the lesion number produced by the Colletotrichum species but did reduce basal resistance to P. syringae pv. tabaci permitting higher bacterial populations. Based on sequence similarities, PRp27 proteins have been hypothesised to have protease activity and may contribute to resistance by exhibiting direct antimicrobial activity in the apoplast, releasing of antimicrobial compounds from the plant matrix or releasing elicitors from pathogens to induce resistance.
Additional keywords: anthracnose, bacterial wildfire, biotrophy, necrotrophy, pathogenesis-related protein.
Acknowledgements
Pseudomonas syringae pv. tabaci 11528R was kindly provided by Dr G Martin, Boyce Thompson Institute, Cornell University, Ithaca, NY. The plasmid, pEZS-CL, was kindly provided by Dr M Raizada, Department of Plant Agriculture, University of Guelph, Guelph, ON. Funding for this study was provided by the Natural Science and Engineering Research Council of Canada.
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