Isolation of dehydration-responsive genes in a drought tolerant common bean cultivar and expression of a group 3 late embryogenesis abundant mRNA in tolerant and susceptible bean cultivars
Blanca E. Barrera-Figueroa A , Julián M. Peña-Castro A , Jorge A. Acosta-Gallegos B , Roberto Ruiz-Medrano A and Beatriz Xoconostle-Cázares A CA Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. IPN 2508, 07360 San Pedro Zacatenco, México.
B Programa de Mejoramiento del Frijol, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Campo Experimental Bajío. Km. 6.5 carretera Celaya – San Miguel de Allende, 38010 Celaya, Guanajuato, México.
C Corresponding author. Email: bxoconos@cinvestav.mx
D This paper originates from an International Symposium in Memory of Vincent R. Franceschi, Washington State University, Pullman, Washington, USA, June 2006.
Functional Plant Biology 34(4) 368-381 https://doi.org/10.1071/FP06224
Submitted: 9 September 2006 Accepted: 8 February 2007 Published: 19 April 2007
Abstract
Drought is one of the main constraints for common bean (Phaseolus vulgaris L.) production in Latin America. The aim of this work was to identify upregulated genes in the drought-tolerant common bean cv. Pinto Villa, grown under water-deficit conditions. Twenty-eight cDNAs representing differentially-expressed mRNAs in roots and/or leaves were isolated via suppression subtractive hybridisation. Their expression profiles in plants under intermediate and severe dehydration stress were tested. Three cDNAs corresponded to genes already described as associated to drought stress in P. vulgaris, 12 were known P. vulgaris sequences without previous association with drought response, and 13 were new P. vulgaris sequences. Analysis of the deduced proteins encoded by the cDNAs revealed putative functions in cellular protection, sugar metabolism, and protein synthesis, folding and turnover. Additionally, a new member of group 3 late embryogenesis abundant (LEA) genes (PvLEA3) was cloned and its complete sequence was obtained. Given the lack of reports comparing expression of dehydration-responsive genes in bean cultivars with different response to drought, the expression of PvLEA3 transcript in five bean cultivars from different origin was analysed. The induction of PvLEA3 was directly associated with the level of drought tolerance in the cultivars studied.
Additional keywords: drought stress, drought tolerance, late-embryogenesis abundant protein, Phaseolus vulgaris, water deficit.
Acknowledgements
The authors thank our anonymous reviewers for their valuable suggestions. We also thank to A. Monsalvo (UBIPRO-UNAM-Iztacala), B. Jiménez (Langebio-CINVESTAV-Irapuato) for DNA sequencing and C. G. Reynaga-Peña and S. Barrera-Figueroa for critically reading of the preliminary manuscript. BEB-F. and JMP-C. acknowledge doctoral scholarships from CONACyT-México. This research was supported by CONACyT-SAGARPA 27–2004 (BX-C), CONACyT-México 39961 (BX-C), Department of Biotechnology and Bioengineering funds (CINVESTAV-IPN) (BX-C and RR-M) and CONACyT-México 39960 (RR-M).
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