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Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

Cloning and expression analysis of TSK1, a wheat SKP1 homologue, and functional comparison with Arabidopsis ASK1 in male meiosis and auxin signalling

Chijun Li A C , Yu Liang A , Changbin Chen A B , Junhua Li A C , Yunyuan Xu A , Zhihong Xu A , Hong Ma B and Kang Chong A D
+ Author Affiliations
- Author Affiliations

A Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, People’s Republic of China.

B Department of Biology and the Huck Institutes of the Life Sciences, 405D Life Sciences Building, The Pennsylvania State University, University Park, PA 16802, USA.

C Graduate School of the Chinese Academy of Sciences, Beijing 100049, People’s Republic of China.

D Corresponding author. Email: chongk@ibcas.ac.cn

Functional Plant Biology 33(4) 381-390 https://doi.org/10.1071/FP06026
Submitted: 26 January 2006  Accepted: 2 March 2006   Published: 3 April 2006

Abstract

Plants possess multiple homologues of the SKP1 gene encoding an essential subunit of the SCF ubiquitin ligases, but only ASK1 (Arabidopsis SKP1-like 1) and ASK2 have been characterised genetically. In addition, little is known about the function of SKP1 homologues in monocots. Here we report on a winter wheat homologue of SKP1 named TSK1 (Triticum aestivum SKP1-like 1). Expression analyses revealed that it was expressed predominantly in young roots and floral buds. RNA in situ hybridisation showed that it was expressed in the shoot apical meristem (SAM) and anthers, especially the tapetum and microsporocytes at the time of meiosis. It was also expressed in almost the entire meristematic and elongation zones of the root. These observations indicated that TSK1 might function in dividing cells. The Arabidopsis ask1-1 mutant with overexpressed TSK1 driven by the CaMV 35S promoter exhibited partial fertility, suggesting that TSK1 could partially restore function in meiosis to the ask1-1 mutant. In addition, overexpression of TSK1 in wild type Arabidopsis resulted in changes in auxin responses and auxin-related phenotypes, consistent with a role of ASK1 in Arabidopsis auxin response. These results suggest possible functional conservation between TSK1 and ASK1.

Keywords: Arabidopsis, auxin response, meiosis, SKP1, TSK1, wheat.


Acknowledgments

The BA3-GUS seeds were kindly provided by Professor Athanasios Theologis (University of California, Berkeley). This work was supported by the Outstanding Young Research Fund of NSFC (30525026) and innovation grants from the Chinese Academy of Sciences, as well as the Major State Basic Research Program of the People’s Republic of China (2005CB120806).


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