Genetic diversity of Cistus albidus in south-east Spain does not relate to mesoclimate
Olga M. Grant A C D , Tom McNeilly B and Lynton D. Incoll AA School of Biology, University of Leeds, Leeds LS2 9JT, UK.
B School of Biological Sciences, University of Liverpool, Liverpool L69 7ZB, UK.
C Current address: East Malling Research, East Malling, Kent ME19 6BJ, UK.
D Corresponding author. Email: olga.grant@emr.ac.uk
Functional Plant Biology 33(3) 247-255 https://doi.org/10.1071/FP05245
Submitted: 4 October 2005 Accepted: 22 December 2005 Published: 2 March 2006
Abstract
Patterns of variation at selected isozyme loci were examined in populations of Cistus albidus L. in south-east Spain, from areas differing in precipitation and temperature, to determine whether population divergence has occurred. Total genetic diversity was high, with a high percentage of the variation being found between populations. Genetic variation between populations did not relate to climate. Populations exposed to severe summer drought did not show lower genetic diversity than populations from more benign climates. Little genetic variation was found between populations that showed variation in ecophysiological and growth responses in situ and under controlled conditions. Cluster analyses showed different patterns of between-population distances with respect to genetic, physiological and morphological data, and none of these patterns corresponded to that for between-site environmental variation.
Keywords: climatic gradient, genetic variation, isozymes, Mediterranean vegetation, populations, selection.
Acknowledgments
OMG gratefully acknowledges the advice of R Farley and J Keen with respect to enzyme extraction and gel electrophoresis, and the technical assistance of B Evans and H Lewis. OMG thanks the University of Leeds for the award of a postgraduate scholarship. D Appleyard of the Graphics Unit of the School of Geography, University of Leeds, prepared the map of Almería.
Aitken SN, Libby WJ
(1994) Evolution of the pygmy-forest edaphic subspecies of Pinus contorta across an ecological staircase. Evolution 48, 1009–1019.
Balfourier F,
Charmet G, Ravel C
(1998) Genetic differentiation within and between natural populations of perennial and annual ryegrass (Lolium perenne and L. rigidum). Heredity 81, 100–110.
| Crossref | GoogleScholarGoogle Scholar |
Batista F,
Bañares A,
Caujapé-Castells J,
Carqué E,
Marrero-Gómez M, Sosa PA
(2001) Allozyme diversity in three endemic species of Cistus (Cistaceae) from the Canary Islands: intraspecific and interspecific comparisons and implications for genetic conservation. American Journal of Botany 88, 1582–1592.
Blasco S, Mateu I
(1995) Flowering and fruiting phenology and breeding system of Cistus albidus L. Acta Botanica Gallica 142, 245–251.
Bosch J
(1992) Floral biology and pollinators of three co-occurring Cistus species (Cistaceae). Botanical Journal of the Linnean Society 109, 39–55.
Brown AHD
(1979) Enzyme polymorphism in plant populations. Theoretical Population Biology 15, 1–42.
| Crossref | GoogleScholarGoogle Scholar |
Chapin FS,
Autumn K, Pugnaire F
(1993) Evolution of suites of traits in response to environmental stress. American Naturalist 142, S78–S92.
| Crossref | GoogleScholarGoogle Scholar |
Clegg MT, Allard RW
(1972) Patterns of genetic differentiation in the slender wild oat species Avena barbata. Proceedings of the National Academy of Sciences USA 69, 1820–1824.
Correia OA,
Martins AC, Catarino FM
(1992) Comparative phenology and seasonal foliar nitrogen variation in Mediterranean species of Portugal. Ecologia Mediterranea 18, 7–18.
Ehleringer, JR ,
and
Mooney, HA (1983). Productivity of desert and Mediterranean-climate plants. In ‘Encyclopedia of plant physiology, new series’. pp. 205–231. (Springer-Verlag: Berlin)
Farley RA, McNeilly T
(2000) Diversity and divergence in Cistus salvifolius (L.) populations from contrasting habitats. Hereditas 132, 183–192.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
García-Fayos P,
Rectalá TM,
Cerdá A, Calvo A
(1995) Seed population dynamics on badland slopes in southeastern Spain. Journal of Vegetation Science 6, 691–696.
Goudet J,
Raymond M,
de Meeüs T, Rousset F
(1996) Testing differentiation in diploid populations. Genetics 144, 1993–1940.
| PubMed |
Grant OM, Incoll LD
(2005) Photochemical efficiency is an important component of ecophysiological variation of Cistus albidus between habitats in south-east Spain. Functional Plant Biology 32, 107–115.
| Crossref | GoogleScholarGoogle Scholar |
Grant OM,
Incoll LD, McNeilly T
(2005) Variation in growth responses to availability of water in Cistus albidus populations from differing climates. Functional Plant Biology 32, 817–829.
| Crossref | GoogleScholarGoogle Scholar |
Hamrick, JL ,
and
Godt, MJW (1989). Allozyme diversity in plant species. In ‘Plant population genetics, breeding and genetic resources’. pp. 43–63. (Sinauer: Sunderland, MA)
Heywood JS, Levin DA
(1985) Associations between allozyme frequencies and soil characteristics in Gaillardia pulchella (Compositae). Evolution 39, 1076–1086.
Lack AJ, Kay QON
(1988) Allele frequencies, genetic relationships and heterozygosity in Polygala vulgaris populations from contrasting habitats in southern Britain. Biological Journal of the Linnean Society 34, 119–147.
Lázaro R, Rey JM
(1991) Sobre el clima de la provincia de Almería (SE Iberico): Primer ensayo de cartografia automatica de medias anuales de temperatura y precipitacion. Suelo y Planta 1, 61–68.
Le Houérou, HN (1990). Global change: population, land use and vegetation in the Mediterranean Basin by the mid-21st century. In ‘Greenhouse effect, sea-level and drought’. pp. 301–367. (Kluwer Academic: Dordrecht)
Lundkvist K
(1979) Allozyme frequency distributions in four Swedish populations of Norway spruce (Picea abies K.) I. Estimations of genetic variation within and among populations, genetic linkage and a mating system parameter. Hereditas 90, 127–143.
Nei M
(1972) Genetic distance between populations. American Naturalist 106, 283–292.
| Crossref | GoogleScholarGoogle Scholar |
Nei M
(1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences USA 70, 3321–3323.
Nevo E
(2001) Evolution of genome-phenome diversity under environmental stress. Proceedings of the National Academy of Sciences USA 98, 6233–6240.
| Crossref | GoogleScholarGoogle Scholar |
Nevo E,
Zohary D,
Brown AHD, Haber M
(1979) Genetic diversity and environmental associations of wild barley, Hordeum spontaneum, in Israel. Evolution 33, 815–833.
Nevo E,
Beiles A, Kaplan D
(1988) Genetic diversity and environmental associations of wild emmer wheat, in Turkey. Heredity 61, 31–45.
Norušis, MJ (1994).
Raymond M, Rousset F
(1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. The Journal of Heredity 86, 248–249.
Rousset F, Raymond M
(1995) Testing heterozygote excess and deficiency. Genetics 140, 1413–1419.
| PubMed |
Shaw CR, Prasad R
(1970) Starch gel electrophoresis of enzymes — a compilation of recipes. Biochemical Genetics 4, 297–320.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Soltis DE,
Haufler CH,
Darrow DC, Gastony JG
(1983) Starch gel electrophoresis of ferns: a compilation of grinding buffers, gel and electrode buffers, and staining schedules. American Fern Journal 73, 9–27.
Sultan SE
(1996) Phenotypic plasticity for offspring traits in Polygonum persicaria. Ecology 77, 1791–1807.
Sun G-L,
Díaz S,
Alomon B, Von Bothmer R
(1998) Microsatellite variation and its comparison with allozyme and RAPD variation in Elymus fibrosus (Schrenk) Tzvel. (Poaceae). Hereditas 129, 275–282.
| Crossref | GoogleScholarGoogle Scholar |
Thanos CA, Georghiou K
(1988) Ecophysiology of fire-stimulated seed germination in Cistus incanus ssp. creticus (L.) and C. salvifolius L. Plant, Cell & Environment 11, 841–849.
Tutin, TG (1968).
Valladares F,
Balaguer L,
Martinez-Ferri E,
Perez-Corona E, Manrique E
(2002) Plasticity, instability and canalization: is the phenotypic variation in seedlings of sclerophyll oaks consistent with the environmental unpredictability of Mediterranean ecosystems? New Phytologist 156, 457–467.
| Crossref | GoogleScholarGoogle Scholar |
Vanhala TK,
Rijn CPE,
Buntjer J,
Stam P,
Nevo E,
Poorter H, van Eeuwijk FA
(2004) Environmental, phenotypic and genetic variation of wild barely (Hordeum spontaneum) from Israel. Euphytica 137, 297–309.
| Crossref | GoogleScholarGoogle Scholar |
van Kleunen M, Fischer M
(2005) Constraints on the evolution of adaptive phenotypic plasticity in plants. New Phytologist 166, 49–60.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
van Kleunen M, Ritland K
(2005) Estimating heritabilities and genetic correlations with marker-based methods: an experimental test in Mimulus guttatus. The Journal of Heredity 96, 368–375.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Verspoor E,
Beardmore JA,
Consuegra S,
De Leaniz CG, Hindar K , et al.
(2005) Population structure in the Atlantic salmon: insights from 40 years of research into genetic protein variation. Journal of Fish Biology 67([Suppl. A.]), 3–54.
| Crossref | GoogleScholarGoogle Scholar |
Waldmann P,
Garcia-Gil MR, Sillanpaa MJ
(2005) Comparing Bayesian estimates of genetic differentiation of molecular markers and quantitative traits: an application to Pinus sylvestris. Heredity 94, 623–629.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Westerbergh A, Saura A
(1992) The effect of serpentine on the population structure of Silene dioica (Caryophyllaceae). Evolution 46, 1537–1548.
Zoro Bi I,
Maquet A, Baudoin J-P
(1997) Spatial patterns of allozyme variants within three wild populations of Phaseolus lunatus L. from the central valley of Costa Rica. Belgian Journal of Botany 129, 149–155.
