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RESEARCH ARTICLE
Contents Vol 33(1)

TaNAC69 from the NAC superfamily of transcription factors is up-regulated by abiotic stresses in wheat and recognises two consensus DNA-binding sequences

Gang-Ping Xue A C , Neil I. Bower A B , C. Lynne McIntyre A , George A. Riding B , Kemal Kazan A and Ray Shorter A
+ Author Affiliations
- Author Affiliations

A CSIRO Plant Industry, 306 Carmody Rd, St Lucia, Qld 4067, Australia.

B CSIRO Livestock Industries, 306 Carmody Rd, St Lucia, Qld 4067, Australia.

C Corresponding author. Email: Gang-Ping.Xue@csiro.au

Functional Plant Biology 33(1) 43-57 https://doi.org/10.1071/FP05161
Submitted: 1 July 2005  Accepted: 16 August 2005   Published: 3 January 2006

Abstract

NAC proteins are one of the largest families of plant transcription factors and have recently been implicated in diverse physiological processes. To elucidate their role in gene regulation, we determined the DNA-binding specificity of a drought- and cold-inducible NAC protein, TaNAC69 from wheat, and analysed its homologues from other species. Two consensus DNA-binding sequences (spanning 23–24 bp) of TaNAC69 were identified through binding site selection and both consisted of two half sites. Comprehensive data on the DNA-binding specificity of TaNAC69 were generated through extensive base substitution mutagenesis. TaNAC69 and its homologue in Arabidopsis, NAP, sharing 75% sequence identity in the NAC domain, exhibited similar DNA-binding specificity. TaNAC69 was able to homodimerise through its NAC domain. The NAC domain consists of five conserved subdomains. Subdomain mutation showed that a loss or reduction in TaNAC69 dimerisation capacity was accompanied with abolition or decrease in its DNA-binding activity. These data suggest that all subdomains are necessary to maintain a functional NAC domain structure required for interaction with DNA and dimerisation.

Keywords: Arabidopsis, dimerisation, DNA-binding specificity, drought and cold stress, NAC transcription factor, wheat.


Acknowledgment

The project was supported by the Australian Grains Research and Development Corporation.


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