Changes in photosynthetic parameters and antioxidant activities following heat-shock treatment in tomato plants
Daymi Camejo A , Ana Jiménez B C , Juan José Alarcón B , Walfredo Torres A , Juana María Gómez B and Francisca Sevilla BA Instituto Nacional de Ciencias Agrícolas, INCA, Gaveta Postal 1, 23700, San José de las Lajas, La Habana, Cuba.
B Centro de Edafología y Biología Aplicada del Segura, CSIC, Apartado 164, E-30100 Murcia, Spain.
C Corresponding author. Email: ajimenez@cebas.csic.es
Functional Plant Biology 33(2) 177-187 https://doi.org/10.1071/FP05067
Submitted: 17 March 2005 Accepted: 15 September 2005 Published: 3 February 2006
Abstract
Seedlings of two tomato genotypes, Lycopersicon esculentum Mill. var. Amalia and the wild thermotolerant type Nagcarlang, were grown under a photoperiod of 16 h light at 25°C and 8 h dark at 20°C. At the fourth true leaf stage, a group of plants were exposed to a heat-shock temperature of 45°C for 3 h, and measurements of chlorophyll fluorescence, gas-exchange characteristics, dark respiration and oxidative and antioxidative parameters were made after releasing the stress. The heat shock induced severe alterations in the photosynthesis of Amalia that seem to mitigate the damaging impact of high temperatures by lowering the leaf temperature and maintaining stomatal conductance and more efficient maintenance of antioxidant capacity, including ascorbate and glutathione levels. These effects were not evident in Nagcarlang. In Amalia plants, a larger increase in dark respiration also occurred in response to heat shock and the rates of the oxidative processes were higher than in Nagcarlang. This suggests that heat injury in Amalia may involve chlorophyll photooxidation mediated by activated oxygen species (AOS) and more severe alterations in the photosynthetic apparatus. All these changes could be related to the more dramatic effect of heat shock seen in Amalia than in Nagcarlang plants.
Keywords: antioxidant activities, fluorescence, heat shock, photosynthesis, tomato.
Acknowledgments
This work was supported by grants from the ‘Convenio de Cooperación Científica Hispano-Cubano del CSIC/CITMA’ (2001CU0015), and the MCYT-FEDER, Ministry of Science and Technology (BFI 2002-03207).
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