Low temperature effects on grapevine photosynthesis: the role of inorganic phosphate
Luke Hendrickson A , Wah Soon Chow A and Robert T. Furbank B CA Research School of Biological Sciences, Australian National University, GPO Box 475, Canberra, ACT 2601, Australia.
B CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia.
C Corresponding author; email: robert.furbank@csiro.au
Functional Plant Biology 31(8) 789-801 https://doi.org/10.1071/FP04037
Submitted: 16 February 2004 Accepted: 20 May 2004 Published: 23 August 2004
Abstract
The photosynthetic response of grapevine leaves (Vitis vinifera L. cv. Riesling) to low temperature was studied to determine the role of end-product limitation and orthophosphate (Pi) recycling to the chloroplast under these conditions. As reported previously, the response of photosynthesis in air to stomatal conductance declined at temperatures below 15°C, suggesting that at low temperatures inhibition of photosynthesis in grapevine has a strong non-stomatal component. Stimulation of carbon assimilation at ambient CO2 by reducing O2 from 21 to 2 kPa, O2 declined to zero below 15°C, a phenomenon often associated with a restriction in photosynthesis due to end-product-synthesis limitation. This stimulation could be restored by feeding Pi. Photosynthesis in leaf disks at both high and low irradiances in non-photorespiratory conditions (1% CO2) was highly sensitive to reductions in temperature. Below 15°C, feeding Pi caused a large stimulation of photosynthetic O2 evolution. Metabolite measurements indicated that despite a decline in Rubisco carbamylation state, ribulose 1,5-bisphosphate (RuBP) levels dropped at low temperature and the ratio of 3-phosphoglycerate (3-PGA) to triose phosphate (TP) remained largely unchanged. These results suggest that grapevine-leaf photosynthesis is severely restricted at low temperature by non-stomatal mechanisms. The return of Pi to the chloroplast plays an important role in this limitation but a coordinated set of regulatory processes maintain a homeostasis of phosphorylated sugar levels.
Keywords: chilling, end-product limitation, low temperature, phosphate, photosynthesis, Vitis vinifera.
Acknowledgments
We acknowledge Prof. Barry Osmond for establishing the ARC Strategic Partnerships with Industry-Research and Training Scheme Grant (C19906986) that funded this study.
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