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RESEARCH ARTICLE
Contents Vol 31(10)

Nitrogen source and water regime effects on barley photosynthesis and isotope signature

Marta S. Lopes A , Salvador Nogués A and José L. Araus A B
+ Author Affiliations
- Author Affiliations

A Unitat de Fisiologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, E-08 028 Barcelona, Spain.

B Corresponding author; email: jaraus@ub.edu

Functional Plant Biology 31(10) 995-1003 https://doi.org/10.1071/FP04031
Submitted: 16 February 2004  Accepted: 20 July 2004   Published: 14 October 2004

Abstract

Water stress and nitrogen (N) availability are the main constraints on barley (Hordeum vulgare L.) yield in Mediterranean conditions. Here we studied the combined effects of N source and water regime (WR) on plant growth, photosynthesis and carbon isotope discrimination (Δ13C) in barley grown under controlled conditions. The effects of these conditions on plant N isotope discrimination against the fertiliser (Δ15N) was also examined to assess whether the natural variation in plant N isotope composition is a reliable indicator of N nutrition. Six experimental treatments were established with three nutrient solutions containing ammonium (NH4+), nitrate (NO3–) or a mixture of the two (NH4+ : NO3–), each either well watered or moderately water stressed. The NH4+ : NO3– treatment resulted in the greatest biomass accumulation and photosynthetic capacity in both WRs. The NH4+ plants showed accelerated phenology and depressed growth. They also had the lowest photosynthetic rates in both WRs. This effect was mainly due to stomatal closure, while electron transport and carboxylation capacity of leaves were less affected. Consistent with lower stomatal conductance, leaf Δ13C was lower in plants that received NH4+, indicating higher water use efficiency (WUE) not only when irrigated, but also under water stress. In addition, leaf Δ13C and photosynthetic N use efficiency (PNUE) correlated positively with each other and with shoot biomass in both WRs. However, NO3– treatment produced the greatest Δ15N, which was higher in leaves than in roots. Leaf Δ15N was decreased by water stress only in plants in the NO3– treatment. We conclude that leaf Δ13C is an adequate trait to assess the differences in growth, photosynthetic activity and WUE caused by distinct N sources. However, the usefulness of natural abundance of 15N in plant tissue as a nitrogen source marker is restricted by the effect of WR and internal plant fractionation, at least for plants that received NO3–.

Keywords: ammonium, Hordeum vulgare, nitrate, water stress, water use efficiency.


Acknowledgments

This study was supported in part by the EC project MABDE (ICA3-CT-2002–10026). M Lopes was the recipient of a predoctoral fellowship sponsored by the ‘Fundaçao para a Ciência e a Tecnologia’, Portugal.


References


Acevedo E (1993) Potential of carbon isotope discrimination as a selection criterion in barley breeding. ‘Stable isotopes and plant carbon water relations’. (Eds JR Ehleringer, GD Hall) pp. 399–417. (Academic Press: New York)

Ali A, Tucker TC, Thompson TL, Salim M (2001) Effects of salinity and mixed ammonium nitrate nutrition on the growth and nitrogen utilisation of barley. Journal of Agriculture and Crop Science 186, 223–228.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Andrews JR, Bredenkamp GJ, Baker NR (1993) Evaluation of the role of state transitions in determining the efficiency of light utilisation for CO2 assimilation in leaves. Photosynthesis Research 38, 15–16. open url image1

Araus JL, Febrero A, Buxó R, Camalich MD, Martín D, Molina F, Rodríguez-Ariza MO, Romagosa I (1997) Changes in carbon isotope discrimination in grain cereals from different regions of the western Mediterranean Basin during the past seven millennia. Palaeoenvironmental evidence of a differential change in aridity during the late Holocene. Global Change Biology 3, 107–118.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Araus JL, Slafer GA, Reynolds MP, Royo C (2002) Plant breeding and drought in C3 cereals: what should we breed for? Annals of Botany 89, 925–940.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Arnau G, Monneveux P, This D, Alegre L (1997) Photosynthesis of six barley genotypes as affected by water stress. Photosynthetica 34, 67–76.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Basra AS, Goyal SS (2002) Mechanisms of improved nitrogen-use efficiency in cereals. 18. ‘Quantitative genetics, genomics and plant breeding’. (Ed. MS Kang) pp. 269–288. (CAB International: Louisiana State University)

Bort J, Araus JL, Hazzan H, Grando S, Ceccarelli S (1998) Relationships between early vigour, grain yield, leaf structure and stable isotope composition in field grown barley. Plant Physiology and Biochemistry 36, 889–897.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Bort J, Febrero A, Amaro T, Araus JL (1994) Role of awns in ear water-use efficiency and grain weight in barley. Agronomie 2, 133–139. open url image1

Botella MAA, Cruz C, Loução MM, Cerdá A (1993) Nitrate reductase activity in wheat seedlings as affected by NO3 – / NH4 + ratio and salinity. Journal of Plant Physiology 142, 531–536. open url image1

Britto DT, Kronzucker HJ (2002) NH4+ toxicity in higher plants: a critical review. Journal of Plant Physiology 159, 567–584. open url image1

Condon AG, Richards RA, Farquhar GD (1987) Carbon isotope discrimination is positively correlated with grain yield and dry matter production in field grown wheat. Crop Science 27, 996–1001. open url image1

Cornic G, Fresneau C (2002) Photosynthetic carbon reduction and carbon oxidation cycles are the main electron sinks for photosystem II activity during a mild drought. Annals of Botany 89, 887–894.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Cox WJ, Reisenauer HM (1973) Growth and ion uptake by wheat supplied nitrogen as nitrate, or ammonium, or both. Plant and Soil 38, 363–380. open url image1

Cramer MD, Lewis OAM (1993) The influence of nitrate and ammonium nutrition on the growth of wheat (Triticum aestivum) and maize (Zea mays) plant. Annals of Botany 72, 359–365.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Dawson TE, Mambelli S, Plamboeck AH, Templer PH, Tu KP (2002) Stable isotopes in plant ecology. Annual Review of Ecology and Systematics 33, 507–559.
| Crossref |
open url image1

Evans LT (1989) Photosynthesis and nitrogen relationships in leaves of C3 plants. Oecologia 78, 9–19. open url image1

Evans RD (2001) Physiological mechanisms influencing plant nitrogen isotope composition. Trends in Plant Science 6, 121–126.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Evans RD, Bloom AJ, Sukrapana SS, Egleringer JR (1996) Nitrogen isotope composition of tomato (Lycopersicon esculentum Mill cv. T-5) grown under ammonium or nitrate nutrition. Plant, Cell and Environment 19, 1317–1323. open url image1

Farquhar GD, Ehleringer JR, Hubick KT (1989) Carbon isotope discrimination and photosynthesis. Annual Review of Plant Physiology and Plant Molecular Biology 40, 503–537.
| Crossref |
open url image1

Farquhar GD, Richards RA (1984) Isotopic composition of plant carbon correlates with water use efficiency of wheat genotypes. Australian Journal of Plant Physiology 11, 539–552. open url image1

Febrero A, Fernández S, Molina-Cano JL, Araus JL (1998) Yield, carbon isotope discrimination, canopy reflectance and cuticular conductance of barley isolines of differing glaucousness. Journal of Experimental Botany 49, 1575–1581.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Foyer CH, Valadier MH, Migge A, Becker TW (1998) Drought-induced effects on nitrate reductase activity and mRNA and on the concentration of nitrogen and carbon metabolism in maize leaves. Plant Physiology 117, 283–292.
| Crossref | PubMed |
open url image1

Genty D, Briantais JM, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochemica et Biophysica Acta 990, 87–92. open url image1

Handley LL, Odee D, Scrimgeour CM (1994) δ15N and δ13C patterns in savanna vegetation: dependence on water availability and disturbance. Functional Ecology 8, 306–314. open url image1

Handley LL, Austin AT, Robinson D, Scrimgeour CM, Raven JA, Heaton TH, Schmidt S, Stewart GR (1999) The 15N natural abundance (δ15N) of ecosystem samples reflects measures of water availability. Australian Journal of Plant Physiology 26, 185–199. open url image1

Högberg P (1997) 15N natural abundance in soil–plant systems. New Phytologist 137, 179–203.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Horton F, Ruban AV, Walters RG (1996) Regulation of light harvesting in green plants. Annual Review of Plant Physiology and Plant Molecular Biology 47, 655–684.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Hubick KT, Farquhar GD (1989) Carbon isotope discrimination and the ratio of carbon gained to water lost in barley cultivars. Plant, Cell and Environment 12, 795–804. open url image1

Kirda C, Mohamed KS, Kumarsinghe A, Montenegro ZF (1992) Carbon isotope discrimination at vegetative stage as an indicator of yield and water use efficiency of spring wheat (Triticum turgidum L. var durum) Plant and Soil 7, 217–223. open url image1

Kolb KJ, Evans RD (2002) Implications of leaf nitrogen recycling on the nitrogen isotope composition of deciduous plant tissues. New Phytologist 156, 57–64.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Kolb KJ, Evans RD (2003) Influence of nitrogen source and concentration on nitrogen isotopic discrimination in two barley genotypes (Hordeum vulgare L.). Plant, Cell and Environment 26, 1431–1440.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Laporte MM, Shen B, Tarczynski MC (2002) Engineering for drought avoidance, expression of Maize NADP-malic enzyme in tobacco results in altered stomatal function. Journal of Experimental Botany 53, 699–705.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Lawlor D (2002) Carbon and nitrogen assimilation in relation to yield, mechanisms are the key to understanding production systems. Journal of Experimental Botany 53, 773–787.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Lawlor DW, Cornic G (2002) Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant, Cell and Environment 25, 275–294.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Loreto F, Harley PC, Di Marco G, Sharkey TD (1992) Estimation of mesophyll conductance to carbon dioxide flux by three different methods. Plant Physiology 98, 1437–1443. open url image1

Marschner, H (1995). ‘Mineral nutrition of higher plants (2nd edn).’ (Academic Press: London)

Martínez-Carrasco R (1998) Regulation of growth, water use efficiency, and δ13C by the nitrogen source in Casuarina equisetifolia Forst. and Forst. Plant and Cell Environment 21, 531–534.
| Crossref |
open url image1

Munjal N, Sawhney SK, Sawhney V (1997) Activation of nitrate reductase in extracts of water stressed wheat. Phytochemistry 45, 659–665.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Nogués S, Alegre L (2002) An increase in water deficit has no impact on the photosynthetic capacity of field-grown Mediterranean plants. Functional Plant Biology 29, 621–630.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Nogués S, Baker NR (2000) Effects of drought on photosynthesis in Mediterranean plants grown under enhanced UV-B radiation. Journal of Experimental Botany 51, 1309–1317.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Oweis T, Pala M, Ryan J (1998) Stabilizing rainfed wheat yields with supplemental irrigation and nitrogen in a Mediterranean climate. Agronomy Journal 90, 672–681. open url image1

Oweis T, Zhang H, Pala M (2000) Water use efficiency of rainfed and irrigated bread wheat in a Mediterranean environment. Agronomy Journal 92, 231–238.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Passioura JB (2002) Environmental biology and crop improvement. Functional Plant Biology 29, 537–546.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Robinson D (2001) δ15N as an integrator of the nitrogen cycle. Trends in Ecology & Evolution 16, 153–162.
| Crossref | PubMed |
open url image1

Robinson D, Handley LL, Srimgeour CM (1998) A theory for 15N / 14N fractionation in nitrate-grown vascular plants. Planta 205, 397–406.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Robinson D, Handley LL, Srimgeour CM, Gordon DC, Forster BP, Ellis RP (2000) Using stable isotope natural abundance (δ15N and δ13C) to integrate the stress responses of wild barley (Hordeum spontaneoum C. Koch.) genotypes. Journal of Experimental Botany 51, 41–50.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Schulze ED, Williams RJ, Farquhar GD, Schulze W, Langridge J, Miller JM, Walker BH (1998) Carbon and nitrogen isotope discrimination and nitrogen nutrition of trees along a rainfall gradient in northern Australia. Australian Journal of Plant Physiology 25, 413–425.
| Crossref |
open url image1

Smith DI, Dijak M, Bulman P, Ma BL, Hamel C (1998) Barley: physiology of yield. ‘Crop yield. Physiology and processes’. (Eds DL Smith, C Hamel) pp. 67–107. (Springer-Verlag: Berlin)

Vincent R, Fraisier V, Chaillou S, Limami MA, Deleend E, Philipson B, Douat C, Boutin JP, Hirel B (1997) Overexpression of a soybean gene encoding cytosolic glutamine synthetase in shoots of transgenic Lotus cormniculatus L. plants triggers changes in ammonium assimilation and plant development. Planta 201, 424–433.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Wang XT, Below FE (1996) Tillering, nutrient accumulation, and yield of winter wheat as influenced by nitrogen form. Journal of Plant Nutrition 18, 1177–1189. open url image1

Werner R, Schmidt HL (2002) The in vivo nitrogen isotope discrimination among organic plant compounds. Phytochemistry 61, 465–484.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Yin ZH, Raven JA (1998) Influences of different nitrogen sources on nitrogen and water use efficiency, and carbon isotope discrimination, in C3 Triticum aestivum L. and C4 Zea mays L. plants. Planta 205, 574–580.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Yoneyama T, Handley LL, Scrimgeour CM, Fisher DB, Raven JA (1997) Variations of the natural abundances of nitrogen and carbon isotopes in Triticum aestivum, with special reference to phloem and xylem exudates. New Phytologist 137, 205–213.
| Crossref | GoogleScholarGoogle Scholar | open url image1