A ten-year study on the physiology of two Spanish grapevine cultivars under field conditions: effects of water availability from leaf photosynthesis to grape yield and quality

Abstract

The effects of moderate irrigation, compared with non-irrigation, on leaf photosynthesis and transpiration, grape yield, and quality parameters, were studied over ten years in two Spanish cultivars (Tempranillo and Manto Negro) of field-grown grapevines (Vitis vinifera L.). The aim was to increase our knowledge of the relationships between water availability, canopy water losses, photosynthesis, and fruit yield and quality. A second aim was to analyse some of the mechanisms of photosynthetic down-regulation under drought, such as the capacity for RuBP regeneration and Rubisco activity.

Moderate irrigation improved plant water status, leaf photosynthesis and transpiration. Considering the results over ten years, soil water availability (estimated as pre-dawn leaf water potential, Ψ_PD) largely determined leaf photosynthesis and leaf transpiration. Decreased photosynthesis was due to both stomatal and non-stomatal factors. The latter were related to decayed electron transport rate and reduced RuBP regeneration capacity, but not to decreased Rubisco activity.

Moderate irrigation also improved grape yield, although this effect was much larger in Tempranillo than in Manto Negro. Moreover, the correlation between photosynthesis and grape yield was significant in Tempranillo, but not in Manto Negro. In contrast, the correlation between Ψ_PD and several parameters reflecting fruit quality (such as soluble solids and total polyphenol content) was significant only in Manto Negro. These results suggest that there is a close link between water availability and grape yield, mostly through water stress effects on photosynthesis. Drought effects on grape quality are linked to water availability but not to photosynthesis or yield.