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Australasian Plant Disease Notes Australasian Plant Disease Notes Society
RESEARCH ARTICLE

First report of Squash leaf curl China virus in Pakistan

M. Tahir A D , M. S. Haider B and R. W. Briddon C
+ Author Affiliations
- Author Affiliations

A NUST Centre of Virology and Immunology, National University of Science and Technology, Tamiz ud Din Road, Lalkurti, Rawalpindi, Pakistan.

B School of Biological Sciences, University of the Punjab, Lahore, Pakistan.

C National Institute for Biotechnology and Genetic Engineering, Jhang Road, Faisalabad, Pakistan.

D Corresponding author. Email: gullsbs@yahoo.com

Australasian Plant Disease Notes 5(1) 21-24 https://doi.org/10.1071/DN10009
Submitted: 5 January 2010  Accepted: 22 February 2010   Published: 22 March 2010

Abstract

Leaf samples of Cucurbita pepo plants showing yellow mosaic symptoms were collected from Lahore, Pakistan. Full-length clones of a bipartite begomovirus were isolated and sequenced in their entirety. The sequences showed the highest levels of nucleotide sequence identity (98.4% for the DNA-A and 89.6% for the DNA-B) to the ‘Indian’ strain of Squash leaf curl China virus (SLCCNV).


Acknowledgement

M. Tahir and M. S. Haider acknowledge the support of the School of Biological Sciences, University of the Punjab, in conducting this study. R. W. Briddon is supported by the Higher Education Commission (Pakistan) under the ‘‘Foreign Faculty Hiring’’ scheme.


References


Ali A, Natsuaki T, Okuda S (2004) Identification and molecular characterization of viruses infecting cucurbits in Pakistan. Journal of Phytopathology 152, 677–682.
Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Böttcher B, Unseld S, Ceulemans H, Russell RB, Jeske H (2004) Geminate structures of African cassava mosaic virus. Journal of Virology 78, 6758–6765.
Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Briddon RW, Stanley J (2006) Sub-viral agents associated with plant-infecting single-stranded DNA viruses. Virology 344, 198–210.
Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus (San Francisco, Calif.) 12, 13–15. open url image1

Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X (2008) Geminivirus strain demarcation and nomenclature. Archives of Virology 153, 783–821.
Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Hanley-Bowdoin L, Settlage SB, Orozco BM, Nagar S, Robertson D (1999) Geminiviruses: Models for plant DNA replication, transcription, and cell cycle regulation. Critical Reviews in Plant Sciences 18, 71–106.
Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Hussain M, Mansoor S, Iram S, Zafar Y, Briddon RW (2004) First report of Tomato leaf curl New Delhi virus affecting chilli pepper in Pakistan. Plant Pathology 53, 794.
Crossref | GoogleScholarGoogle Scholar | open url image1

Jones DR (2003) Plant viruses transmitted by whiteflies. European Journal of Plant Pathology 109, 195–219.
Crossref | GoogleScholarGoogle Scholar | open url image1

Mansoor S, Hussain M, Khan SH, Bashir A, Leghari AB, Panwar GA, Siddiqui WA, Zafar Y, Malik KA (1998) Polymerase chain reaction-based detection of cotton leaf curl and other whitefly-transmitted geminiviruses from Sindh. Pakistan Journal of Biological Sciences 1, 39–43.
Crossref | GoogleScholarGoogle Scholar | open url image1

Page RDM (1996) TREEVIEW: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12, 357–358.
CAS | PubMed |
open url image1

Revill PA, Ha CV, Porchun SC, Vu MT, Dale JL (2003) The complete nucleotide sequence of two distinct geminiviruses infecting cucurbits in Vietnam. Archives of Virology 148, 1523–1541.
Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Singh R, Raj SK, Prasad V (2008) Molecular characterization of a strain of Squash leaf curl China virus from north India. Journal of Phytopathology 156, 222–228.
Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The Clustal X windows interface; flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876–4882.
Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Zhang W, Olson NH, Baker TS, Faulkner L, Agbandje-McKenna M, Boulton MI, Davies JW, McKenna R (2001) Structure of the maize streak virus geminate particle. Virology 279, 471–477.
Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1