Free Standard AU & NZ Shipping For All Book Orders Over $80!
Register      Login
Australasian Plant Disease Notes Australasian Plant Disease Notes Society
RESEARCH ARTICLE

First report of a ‘Candidatus Phytoplasma cynodontis’-related strain (group 16SrXIV) associated with white leaf disease of Dichanthium annulatum in India

G. P. Rao A C , S. Mall A , M. Singh A and C. Marcone B
+ Author Affiliations
- Author Affiliations

A Sugarcane Research Station, Kunraghat, Gorakhpur 273 008, Uttar Pradesh, India.

B Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, Via Ponte Don Melillo, I-84084 Fisciano (Salerno), Italy.

C Corresponding author. Email: gprao_gor@rediffmail.com

Australasian Plant Disease Notes 4(1) 56-58 https://doi.org/10.1071/DN09023
Submitted: 14 October 2008  Accepted: 17 April 2009   Published: 15 June 2009

Abstract

A phytoplasma has been detected in a formerly undescribed white leaf disease of Dichanthium annulatum (Kleberg’s bluestem) in India. By sequence and phylogenetic analyses of polymerase chain reaction-amplified rDNA sequences, the detected phytoplasma proved to be closely related to Bermuda grass white leaf agent (‘Candidatus Phytoplasma cynodontis’) which is a member of the 16SrXIV group.


References


Ahrens U, Seemüller E (1992) Detection of DNA of plant pathogenic mycoplasmalike organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. Phytopathology 82, 828–832.
Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Deng S, Hiruki C (1991) Amplification of 16S rRNA genes from culturable and nonculturable mollicutes. Journal of Microbiological Methods 14, 53–61.
Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Gundersen DE, Lee I-M (1996) Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathologia Mediterranea 35, 144–151.
CAS |
open url image1

IRPCM Phytoplasma/Spiroplasma Working Team – Phytoplasma taxonomy group (2004) ‘Candidatus Phytoplasma’, a taxon for the wall-less, non-helical prokaryotes that colonize plant phloem and insects. International Journal of Systematic and Evolutionary Microbiology 54, 1243–1255.
Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Jung H-Y, Sawayanagi T, Wongkaew P, Kakizawa S, Nishigawa H, Wei W, Oshima K, Miyata S-I, Ugaki M, Hibi T, Namba S (2003) ‘Candidatus Phytoplasma oryzae’, a novel phytoplasma taxon associated with rice yellow dwarf disease. International Journal of Systematic and Evolutionary Microbiology 53, 1925–1929.
Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Marcone C, Schneider B, Seemüller E (2004) ‘Candidatus Phytoplasma cynodontis’, the phytoplasma associated with Bermuda grass white leaf disease. International Journal of Systematic and Evolutionary Microbiology 54, 1077–1082.
Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA 4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 1596–1599.
Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1