First molecular detection and identification of a potyvirus associated with severe mosaic disease of amaryllis (Hippeastrum hybridum Hort.) in India

S. K. Raj; S. K. Snehi; S. Kumar; M. S. Khan

doi:10.1071/DN09021

RESEARCH ARTICLE

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First molecular detection and identification of a potyvirus associated with severe mosaic disease of amaryllis (Hippeastrum hybridum Hort.) in India

S. K. Raj ^A ^B , S. K. Snehi ^A , S. Kumar ^A and M. S. Khan ^A

+ Author Affiliations

- Author Affiliations

^A Plant Molecular Virology, National Botanical Research Institute, Rana Pratap Marg, Lucknow 226 001 (U.P.), India.

^B Corresponding author. Email: skraj2@rediffmail.com

Australasian Plant Disease Notes 4(1) 50-53 https://doi.org/10.1071/DN09021
Submitted: 27 August 2008 Accepted: 4 May 2009 Published: 15 June 2009

Abstract

A potyvirus associated with mosaic disease of amaryllis was detected and identified by reverse-transcription and polymerase chain reaction using degenerate primers specific to the core region of coat protein gene of potyviruses followed by cloning and sequencing. The sequence shared highest (98–95%) amino acid identities with Amaryllis potyvirus that is not yet recognised by the International Committee on Taxonomy of Viruses, Amazon lily mosaic virus (tentative potyvirus) and Hippeastrum mosaic virus (recognised potyvirus). Screening bulbs of elite amaryllis cultivars for potyvirus infection would be useful to establish virus-free propagating material for the floriculture industry in India.

Acknowledgements

The authors express their gratitude to the Director, National Botanical Research Institute, Lucknow for facilities and to Director, Central Institute of Medicinal and Aromatic Plants for electron microscope facilities.

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