First report of a begomovirus infecting the ornamental plant Vinca minor L.
M. Saleem Haider A D E , M. Tahir A , A. Saeed A , S. Ahmed B , R. Parveen C and N. Rashid AA School of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore, Pakistan.
B Institute of Mycology and Plant Pathology, University of the Punjab, Lahore, Pakistan.
C University College of Agriculture, Bahauddin Zakaryia University, Multan, Pakistan.
D Current address: Deptartment of Cell and Systems Biology, University of Toronto, Ontario, Canada.
E Corresponding author. Email: saleem.haider@utoronto.ca
Australasian Plant Disease Notes 3(1) 150-151 https://doi.org/10.1071/DN08058
Submitted: 1 May 2008 Accepted: 8 November 2008 Published: 20 November 2008
Abstract
Vinca minor L., an ornamental plant with symptoms typical of a begomovirus was found in the vicinity of the School of Biological Sciences, Lahore, Pakistan. Symptomatic and apparently healthy leaf samples were collected and subjected to DNA extraction and polymerase chain reaction, using universal primers conserved for the coat protein region of begomoviruses. An expected size product of ~0.78 kb was amplified from symptomatic leaf samples while healthy plant samples gave no amplification. The product was cloned and sequenced in its entirety. Basic Local Alignment Search Tool analysis showed 93% nucleotide sequence identity with Pedilanthus leaf curl virus originating from Pakistan. Our results indicate that this is the first report of a begomovirus infecting V. minor in Pakistan.
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