Apricot is a new host of Apple scar skin viroid

Ying Zhao; Jianxin Niu

doi:10.1071/DN08039

RESEARCH ARTICLE

Previous Next Contents Vol 3(1)

Apricot is a new host of Apple scar skin viroid

Ying Zhao ^A and Jianxin Niu ^A ^B

+ Author Affiliations

- Author Affiliations

^A Horticultural Department, Agricultural College of Shihezi University, Shihezi 832003, China.

^B Corresponding author. Email: njx105@163.com

Australasian Plant Disease Notes 3(1) 98-100 https://doi.org/10.1071/DN08039
Submitted: 7 March 2008 Accepted: 8 July 2008 Published: 17 July 2008

Abstract

Apple scar skin viroid (ASSVd), a pathogenic RNA viroid, infects apple and pear trees. To determine if ASSVd can also infect apricot, we performed reverse transcription–polymerase chain reaction (RT-PCR), spot hybridisation and in situ RT-PCR and detected ASSVd-specific sequences from apricot. We cloned and sequenced the apricot-derived ASSVd and registered it in GenBank. In situ RT-PCR further confirmed the existence of ASSVd in apricot leaf tissues. Our data indicate that apricot is a novel host of ASSVd. These findings provide the basis for the rapid identification of ASSVd in apricot.

Acknowledgements

This work was supported by the National Natural Science Foundation of China program (30360066) and the National Key Technologies R&D Program (2003BA546C).

References

Chen W, Tian P, Jin LP, Wang GP, Liu FC (1986) Study of viroid RNA isolated from apple scar skin disease tissues. Chinese Journal of Virology 2, 79–84.

Guo R (2006) Detection and sequence diversity analysis of viroids isolated from several perennial plants in China. Masters dissertation, Chinese Academy of Agricultural Sciences, Beijing.

Guo R, Li SF, Dong YF, Cheng ZM (2005) Occurrence of Apple scar skin disease and sequence analysis of Apple scar skin viroid in Liaoning. Acta Phytopathologica Sinica 35, 472–474.

Hadidi A, Hansen AJ, Parish CJ, Yang X (1991) Scar skin and dapple apple viroids are seed-borne and persistent in infected apple trees. Research in Virology 142, 289–296.
| Crossref | GoogleScholarGoogle Scholar | PubMed |

Hashimoto J, Koganezawa H (1987) Nucleotide sequence and secondary structure of Apple scar skin viroid. Nucleic Acids Research 15, 7045–7052.
| Crossref | GoogleScholarGoogle Scholar | PubMed |

Koganezawa H, Yanase H, Sakuma T (1983) Viroid-like RNA associated with apple scar skin (or dapple apple) disease. Acta Horticulturae 130, 193–197.

Koganezawa H , Yang X , Zhu SF , Hashimoto J , Hadidi A (2003) Chapter 17. Apple scar skin viroid in apple. In ‘Viroids’. (Eds A Hadidi, R Flores, JW Randles, JS Semancik) pp. 137–141. (CSIRO Publishing: Melbourne)

Kyriakopoulou PE , Osaki H , Zhu SF , Hadidi A (2003) Chapter 18. Apple scar skin viroid in pear. In ‘Viroids’. (Eds A Hadidi, R Flores, JW Randles, JS Semancik) pp. 142–145. (CSIRO Publishing: Melbourne)

Niu JX, Zhou MS, Ma BG, Zhao Y, Liu H (2007) Detection of Apple chlorotic leaf sopt virus in pear by in situ RT-PCR. Acta Horticulturae Sinica 34, 53–58.

Puchta H, Luckinger R, Yang XC, Hadidi A, Saenger HL (1990) Nucleotide sequence and secondary structure of Apple scar skin viroid (ASSVd) from China. Plant Molecular Biology 14, 1065–1067.
| Crossref | GoogleScholarGoogle Scholar | PubMed |

Yang X, Hadidi A, Hammond RW (1992) Nucleotide sequence of Apple scar skin viroid reverse transcribed in host extracts and amplified by the polymerase chain reaction. Acta Horticulturae 309, 305–309.

Zhao Y, Niu JX (2006) Cloning and sequencing of Sinkiang isolate of Apple scar skin viroid (ASSVd). Journal of Fruit Science 23, 896–898.

Zhao Y, Niu JX (2007) Detection of Apple scar skin viroid in pear cultivars by RT-PCR and spot hybridization in Xinjiang area. Journal of Fruit Science 24, 761–764.

Zhu SF, Hadidi A, Hammond RW (1995) Nucleotide sequence and secondary structure of pome fruit viroids from apple disease apples, pear rusty skin diseased pears and apple scar skin symptomless pear. Acta Horticulturae 386, 554–559.