Characterisation of Apple chlorotic leaf spot virus infecting almonds in India
T. Rana A , V. Chandel A , V. Hallan A and A. A. Zaidi A BA Plant Virology Laboratory, Institute of Himalayan Bioresource Technology, Palampur 176 061, Himachal Pradesh, India.
B Corresponding author. Email: zaidi_aijaz@yahoo.com
Australasian Plant Disease Notes 3(1) 65-67 https://doi.org/10.1071/DN08026
Submitted: 5 August 2007 Accepted: 23 April 2008 Published: 5 May 2008
Abstract
Restricted field surveys were conducted to determine the presence and incidence of virus infections in almonds. Representative asymptomatic samples from six locations were tested by enzyme linked immunosorbent assay and reverse transcription and polymerase chain reaction for known stone fruit viruses. Only Apple chlorotic leaf spot virus (ACLSV) infection was detected in one of the six almond samples from the state of Himachal Pradesh. Comparison of sequenced amplicons with two available sequences of ACLSV-almond isolates revealed 92% and 94% amino acid identity. However, the Indian ACLSV-almond isolate shared maximum (98%) homology at the amino acid level to ACLSV-peach (AM498050) and wild apricot (AM498048) isolates from India. The C-terminus is highly conserved within the Indian ACLSV isolates with the exception of a few residues which in the case of the Indian almond isolates are more typical of peach and wild apricot isolate sequences.
Additional Key words: ACLSV, coat protein, RT-PCR.
Acknowledgements
The authors express gratitude to the Director, Institute of Himalayan Bioresource Technology, Palampur (HP), India for encouragement and providing the necessary facilities and to the Department of Science and Technology (Grant no. SR/SO/PS-71/05), Govt. of India.
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