
%0 Journal Article
%A Sainz, RD 
%A Kim, YS 
%A Dunshea, FR 
%A Campbell, RG 
%T Effects of ractopamine in pig muscles: histology, calpains and &#946;-adrenergic receptors
%J Australian Journal of Agricultural Research
%D 1993/10/01
%V 44
%N 7
%P 1441-1448
%K muscles;&#946;-adrenergic agonists;calpains;histology
%R https://doi.org/10.1071/AR9931441
%U https://www.publish.csiro.au/paper/AR9931441 
%X Ractopamine is a G-adrenergic agonist which stimulates growth in pigs, but this effect appears to decline during prolonged treatment. This study was conducted to examine possible mechanisms for the growth-enhancing activity of ractopamine, and its attenuation. Finishing gilts (47.0 kg body weight) were allocated to one of five experimental groups: CON, control diet for 6 weeks; RAC, ractopamine (20 mg/kg feed) for 6 weeks; C/R, CON for 3 weeks and RAC for 3 weeks; R/C, RAC for 3 weeks and CON for 3 weeks; and ALT, CON for weeks 1, 3 and 5, and RAC in weeks 2, 4 and 6. All pigs were fed 2.25 kg/day of a commercial grower ration (13 MJ DE/kg, 16% crude protein, 0.6 g available lysine/MJ DE). Samples of the m. longissirnus dorsi were taken at slaughter and analysed for fibre histology, calpain activities and G -adrenergic receptor populations. Ractopamine had no effect on muscle fibre proportions, but Type II fibres were smaller in pigs treated for the first 3 weeks only (R/C; 2477 8m2, P < 0.05), and tended to be larger in pigs treated for the second 3 weeks only (C/R; 3670 8m2, P < 0.10), as compared to controls (3086 8m2). Giant fibres were also larger in C/R pigs as compared to controls (9027 v. 6917 8m2, P < 0.05). Type I fibres were unaffected by any of the treatments. Calpain II and calpastatin activities were unaffected by any treatment, but calpain I activities were reduced in C/R pigs as compared to controls (0.331 v. 0.504 U/g, P < 0.05), indicating that reduced capacity for protein degradation might partially account for the hypertrophic effect of ractopamine. Pigs treated with ractopamine for 3 weeks before slaughter had reduced numbers of G-adrenoceptors in muscle than controls (2.34 v. 3.17 fmol/mg protein, P < 0.05), which may be responsible for the observed attenuation in growth enhancement over time. 
