
%0 Journal Article
%A Ellison, PJ 
%A Cullis, BR 
%A Bambach, RW 
%A Kable, PF 
%T The effect of temperature on in vitro germination and germ tube growth of urediniospores of <I>Tranzschelia discolor</I>
%J Australian Journal of Agricultural Research
%D 1990/06/01
%V 41
%N 3
%P 479-488
%K 
%R https://doi.org/10.1071/AR9900479
%U https://www.publish.csiro.au/paper/AR9900479 
%X The effect of temperature on in vitro germination and germ tube growth of urediniospores of Tranzschelia discolor was studied over time under constant temperature conditions. Studies were carried out on 1% water agar in the dark at 3¦C, 5¦C, 8¦C, 10¦C, 15¦C, 20¦C, 25¦C, 28¦C, 30¦C and 32¦C. Germination was observed at all temperatures between 5 and 30'C, and occurred rapidly over most of this range. At 2 h, germination exceeded 80% at temperatures between 10 and 28¦C, and this level was reached at 3 h at 8¦C. Germination at 5 and 30¦C was much reduced and at 7 h reached only 44% and 38% respectively. Germ tube growth occurred most vigorously at 15 and 20¦C, reaching lengths in excess of 500 8m at 9 h. The optimum range was narrower than that for germination, and growth was reduced or poor at 8¦C, 10¦C, 25¦C and 28¦C, which were favourable temperatures for germination. Average germ tube lengths at 9 h at these temperatures were 55, 245, 273 and 62 8m, respectively. Three-dimensional models were derived relating germination and germ tube growth to time and temperature. 
