
TY  - JOUR
A1  - Little, DA 
A1  - Lambourne, LJ 
T1  - Changes in the nucleic acid content of the uterus of sheep during involution following ovariectomy and following oestrogenic stimulation
JF  - Australian Journal of Agricultural Research
JO  - Aust. J.  Agric. Res.
Y1  - 1976
VL  - 27
IS  - 5
SP  - 669
EP  - 674
KW  - 
UR  - https://doi.org/10.1071/AR9760669 
N2  - The concentrations of nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), in ovine uterine tissue were studied to determine whether the ratio RNA/DNA might be used in the bioassay of oestrogenic activity. As the uterus decreased in size with time after ovariectomy, the concentration of DNA increased markedly, but the total content of DNA in the uterus remained constant (mean value 342 &plusminus; 8 (SE) mg). The RNA concentration of the tissue decreased during involution, with the result that a highly significant reduction of 33&#37; in the RNA/DNA ratio occurred within 2 weeks of ovariectomy. The ratio decreased further with time. Other ewes were treated for 3 days with diethylstilboestrol dipropionate (10 &#181;g/day), commencing 2 weeks after ovariectomy; the stage of the oestrous cycle at which the ovaries were removed had no effect on the response to the oestrogen. Changes in the nucleic acid concentrations in the uteri of these ewes, and the results obtained during involution, indicate that the RNA/DNA ratio of uterine tissue in ovariectomized ewes has potential as a basis for the bioassay of materials of low oestrogenic potency. 
ER  -

