
TY  - JOUR
A1  - Cheong, J. 
A1  - Wallwork, H. 
A1  - Williams, K. J. 
T1  - Identification of a major QTL for yellow leaf spot resistance in the wheat varieties Brookton and Cranbrook
JF  - Australian Journal of Agricultural Research
JO  - Aust. J.  Agric. Res.
Y1  - 2004
VL  - 55
IS  - 3
SP  - 315
EP  - 319
KW  - Triticum aestivum, disease resistance, microsatellite, marker-assisted selection.
UR  - https://doi.org/10.1071/AR03140 
N2  - Yellow leaf spot (YLS) or tan spot, caused by Pyrenophora tritici-repentis, is a major foliar disease of wheat. A bioassay was used to identify YLS seedling resistance phenotypes of Krichauff/Brookton and Cranbrook/Halberd doubled-haploid (DH) populations. Bulked-segregant analysis was used to identify amplified fragment length polymorphism (AFLP) markers linked to a YLS resistance locus in the wheat cultivar Brookton. Three AFLPs were linked to YLS resistance and also to each other. One of these AFLPs was mapped to the interval Xbcd351&#8211;Xcdo400, which has been assigned to chromosome 5BL. Microsatellite markers in this region were selected from several maps and were genotyped on the Krichauff/Brookton population. Together with the bulked segregant analysis (BSA)-derived AFLPs, the microsatellite markers explained up to 39% of the total phenotypic variation (logarithm of odds ratio, LOD &#8805;  7.32), confirming the chromosome 5BL assignment of a Brookton YLS resistance locus, at or near the previously identified Pyrenophora tritici-repentis toxin-insensitivity locus tsn1. The marker&#8211;trait linkage was validated in the Cranbrook/Halberd DH population, in which the same microsatellite markers explained up to 60% of the total trait variance for YLS. The markers identified can be used for the selection of the Brookton and Cranbrook YLS seedling resistance locus on chromosome 5B. 
ER  -

