
%0 Journal Article
%A Cheong, J. 
%A Wallwork, H. 
%A Williams, K. J. 
%T Identification of a major QTL for yellow leaf spot resistance in the wheat varieties Brookton and Cranbrook
%J Australian Journal of Agricultural Research
%D 2004/03/26
%V 55
%N 3
%P 315-319
%K Triticum aestivum, disease resistance, microsatellite, marker-assisted selection.
%R https://doi.org/10.1071/AR03140
%U https://www.publish.csiro.au/paper/AR03140 
%X Yellow leaf spot (YLS) or tan spot, caused by Pyrenophora tritici-repentis, is a major foliar disease of wheat. A bioassay was used to identify YLS seedling resistance phenotypes of Krichauff/Brookton and Cranbrook/Halberd doubled-haploid (DH) populations. Bulked-segregant analysis was used to identify amplified fragment length polymorphism (AFLP) markers linked to a YLS resistance locus in the wheat cultivar Brookton. Three AFLPs were linked to YLS resistance and also to each other. One of these AFLPs was mapped to the interval Xbcd351&#8211;Xcdo400, which has been assigned to chromosome 5BL. Microsatellite markers in this region were selected from several maps and were genotyped on the Krichauff/Brookton population. Together with the bulked segregant analysis (BSA)-derived AFLPs, the microsatellite markers explained up to 39% of the total phenotypic variation (logarithm of odds ratio, LOD &#8805;  7.32), confirming the chromosome 5BL assignment of a Brookton YLS resistance locus, at or near the previously identified Pyrenophora tritici-repentis toxin-insensitivity locus tsn1. The marker&#8211;trait linkage was validated in the Cranbrook/Halberd DH population, in which the same microsatellite markers explained up to 60% of the total trait variance for YLS. The markers identified can be used for the selection of the Brookton and Cranbrook YLS seedling resistance locus on chromosome 5B. 
