Free Standard AU & NZ Shipping For All Book Orders Over $80!
Register      Login
Australian Journal of Chemistry Australian Journal of Chemistry Society
An international journal for chemical science
RESEARCH FRONT

Bacterial Enzyme Responsive Polymersomes: A Closer Look at the Degradation Mechanism of PEG-block-PLA Vesicles

Katrin-Stephanie Tücking A , Stephan Handschuh-Wang A and Holger Schönherr A B
+ Author Affiliations
- Author Affiliations

A Physical Chemistry I, Faculty of Natural Sciences and Engineering, University of Siegen, Adolf-Reichwein-Str. 2, 57076 Siegen, Germany.

B Corresponding author. Email: schoenherr@chemie.uni-siegen.de

Australian Journal of Chemistry 67(4) 578-584 https://doi.org/10.1071/CH13527
Submitted: 1 October 2013  Accepted: 29 November 2013   Published: 20 January 2014

Abstract

Stimuli-responsive polymersomes that are selectively cleaved by enzymes of pathogenic bacteria are herein reported. The local disruption of the polyester wall in poly(ethylene glycol)-block-poly(lactic acid) (PEG-b-PLA) polymersomes filled with reporter dyes owing to enzymatic degradation by proteinase K was monitored by fluorescence lifetime imaging microscopy. Capsule opening occurred on timescales of minutes to hours, leading to the release of the dye, followed by a slow capsule disintegration, and finally cleavage into monomeric lactic acid over several weeks. These nanocontainers represent a promising element in novel theranostic systems for potential application in advanced wound dressings.


References

[1]  M. Antonietti, S. Förster, Adv. Mater. 2003, 15, 1323.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXntFyjtbk%3D&md5=c32903e2a8f3eda45498faee688286d8CAS |

[2]  L. Luo, A. Eisenberg, Langmuir 2001, 17, 6804.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXnt1aiu7k%3D&md5=41cfd710f575b59679e51feeb48fe6f7CAS |

[3]  Q. Chen, H. Schönherr, G. J. Vancso, Soft Matter 2009, 5, 4944.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhsVyqsr%2FO&md5=ac6babe006805b381a70d1813f83fd4fCAS |

[4]  G. Battaglia, A. J. Ryan, J. Am. Chem. Soc. 2005, 127, 8757.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXks1Sgtro%3D&md5=5025a21db2030eccdb78cda793d1e39fCAS | 15954782PubMed |

[5]  S. Haas, Y. Chen, C. Fuchs, S. Handschuh, M. Steuber, H. Schönherr, Macromol. Symp. 2013, 328, 73.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtFSitLvM&md5=e8c286867312fb62c1cb6060e1f51b85CAS |

[6]  A. Choucair, P. L. Soo, A. Eisenberg, Langmuir 2005, 21, 9308.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXot1Cmu7s%3D&md5=4bc1faffc211a5f2d582d0c46932467bCAS | 16171366PubMed |

[7]  A. Rösler, G. W. M. Vandermeulen, H.-A. Klok, Adv. Drug Deliver. Rev. 2001, 53, 95.
         | Crossref | GoogleScholarGoogle Scholar |

[8]  B. Städler, R. Chandrawati, A. D. Price, S.-F. Chong, K. Breheney, A. Postma, L. A. Connal, A. N. Zelikin, F. Caruso, Angew. Chem. Int. Ed. 2009, 48, 4359.
         | Crossref | GoogleScholarGoogle Scholar |

[9]  A. P. Esser-Kahn, S. A. Odom, N. R. Sottos, S. R. White, J. S. Moore, Macromolecules 2011, 44, 5539.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXosVCqs74%3D&md5=c8922b03b1bf15659dd37c8486195e7bCAS |

[10]  K. J. Zhu, L. Xiangzhou, Y. Shilin, J. Appl. Polym. Sci. 1990, 39, 1.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXosVSjsA%3D%3D&md5=a200cd1e57a0ee5debc73cc3b3b5bb1aCAS |

[11]  F. Meng, Z. Zhong, J. Feijen, Biomacromolecules 2009, 10, 197.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtlartg%3D%3D&md5=566c8bd7ead01ef53a2ea125c79870b8CAS | 19123775PubMed |

[12]  F. Meng, Z. Zhong, J. Phys. Chem. Lett. 2011, 2, 1533.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXntFSksbw%3D&md5=c4651d3713a188ac97ace45750bb65a7CAS |

[13]  E. Cabane, X. Zhang, K. Langowska, C. G. Palivan, W. Meier, Biointerphases 2012, 7, 1.
         | Crossref | GoogleScholarGoogle Scholar |

[14]  F. Meng, C. Hiemstra, G. H. M. Engbers, J. Feijen, Macromolecules 2003, 36, 3004.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXis1Knsbo%3D&md5=5227289dc59694f6540d7ffc9c5e1907CAS |

[15]  A. Belbella, C. Vauthier, H. Fessi, J.-P. Devissguet, F. Puisieux, Int. J. Pharm. 1996, 129, 95.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XitVSru7Y%3D&md5=03c51871d89762d7598ed465468419b0CAS |

[16]  J. Zhou, A. L. Loftus, G. Mulley, A. T. A. Jenkins, J. Am. Chem. Soc. 2010, 132, 6566.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXkvFaqsLc%3D&md5=fb3deba68df470cb3ac04ddba3f7a6edCAS | 20405918PubMed |

[17]  R. E. Drumright, P. R. Gruber, D. E. Henton, Adv. Mater. 2000, 12, 1841.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXntFOqtA%3D%3D&md5=bcebbd4fc97c8ea6c000dffff2e841a3CAS |

[18]  A. Blanazs, S. P. Armes, A. J. Ryan, Macromol. Rapid Comm. 2009, 30, 267.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXivFelu7k%3D&md5=40a27929efcea4defa4e67afde9a5aefCAS |

[19]  Q. Chen, H. Schönherr, G. J. Vancso, Small 2010, 6, 2762.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhsFaitr3K&md5=46d4fafcc724e00255d78d957600fb31CAS | 21072870PubMed |

[20]  H. Schönherr, G. J. Vancso, Scanning Force Microscopy of Polymers 2010 (Springer: Vienna).

[21]  V. Vichaibun, M. Chulavatnatol, ScienceAsia 2003, 29, 297.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXhtVWrs70%3D&md5=7623a42a503ed143a921e4914815e1e6CAS |

[22]  J. Coppeta, C. Rogers, Exp. Fluids 1998, 25, 1.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXjsVOgsb0%3D&md5=9e2fd10badafb9a4ae30e28a84f84c88CAS |

[23]  D. Yan, J. Lu, M. Wei, D. G. Evans, X. Duan, J. Phys. Chem. B 2009, 113, 1381.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXls1eisA%3D%3D&md5=1eae1ba607e751ba1c31963964cd87c2CAS | 19143493PubMed |

[24]  J. R. Lakowicz, Principles of Fluorescence Spectroscopy 2006 (Springer: New York, NY).

[25]  Q. Chen, R. Groote, H. Schönherr, G. J. Vancso, Chem. Soc. Rev. 2009, 38, 2671.
         | Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtVSlsLfL&md5=848f4dc542ac66624486aa0f902949dcCAS | 19690746PubMed |