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RESEARCH ARTICLE
Contents Vol 65(1)

Lipid Peptide Core Nanoparticles as Multivalent Vaccine Candidates against Streptococcus pyogenes

Mariusz Skwarczynski A , Bibi Hamideh Parhiz A , Fatemeh Soltani A , Saranya Srinivasan A , Khairul A. Kamaruzaman A , I-Chun Lin A and Istvan Toth A B
+ Author Affiliations
- Author Affiliations

A The University of Queensland, School of Chemistry and Molecular Biosciences, St. Lucia, Qld 4072, Australia.

B Corresponding author. Email: i.toth@uq.edu.au

Australian Journal of Chemistry 65(1) 35-39 https://doi.org/10.1071/CH11292
Submitted: 15 July 2011  Accepted: 9 September 2011   Published: 3 October 2011

Abstract

Traditional vaccine approaches for Group A streptococcus (GAS) infection are inadequate owing to the host’s production of cross-reactive antibodies that recognize not only the bacteria but also human tissue. To overcome this problem a peptide subunit-based vaccine was proposed, which would incorporate only minimal non-cross reactive epitopes. However, special delivery systems/adjuvants were required because short peptides are not immunogenic. In this study we have incorporated two epitopes from two different GAS proteins into a lipid core peptide (LCP) self-adjuvanting delivery system to achieve better protection against a wide range of GAS serotypes. Multivalent and monovalent constructs were synthesized with the help of an azide alkyne cycloaddition (click) reaction and their ability to self-assemble under aqueous conditions was examined. The compounds significantly differed in their ability to form small size nanoparticles, which are believed to be most appropriate for peptide-based subunit vaccine delivery. The LCP conjugates possessing two different epitopes, in contrast to monoepitopic constructs, formed small nanoparticles (5–15 nm) presumably owing to a suitable hydrophilic-hydrophobic balance of the molecules.


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