The Synthesis of a Two-Photon Fluorescence Labelling Probe and its Immunochromatographic Strip for Rapid Diagnosis of COVID-19
Chibao Huang
A D E , Shuai Kang A , Fuxun Yu B and Zairong Wei C
A School of Physics and Electrical Engineering, Zunyi Normal University, Zunyi 563002, China.
B Centre Laboratory, Guizhou Provincial People’s Hospital, Guiyang 550002, China.
C Affiliated Hospital of Zunyi Medical University, Zunyi Medical University, Zunyi 563000, China.
D Henry Fok School of Biology and Agriculture, Shaoguan University, Shaoguan 512005, China.
E Corresponding author. Email: huangchibao@163.com
Australian Journal of Chemistry 74(7) 522-528 https://doi.org/10.1071/CH20344
Submitted: 26 November 2020 Accepted: 25 January 2021 Published: 25 February 2021
Journal Compilation © CSIRO 2021 Open Access CC BY
Abstract
A two-photon fluorescence labelling probe (LP) was synthesised, and LP-Ag was obtained by LP labelling the N-protein antigen (Ag) of COVID-19. LP-Ag was made into an immunochromatographic strip. When a blood sample was added to the sample hole of the test card, it would move forward along the nitrocellulose (NC) film. If the sample contained IgM, the IgM bound to LP-Ag and formed an M line with the coated mouse anti-human IgM antibody, giving a positive response to the presence of IgM of COVID-19. The sensitivity, specificity, and accuracy of the immunochromatographic strip based on the LP was compared with those of the nucleic acid detection method and the colloidal gold method, proving it to be much simpler than the nucleic acid detection method, which can greatly shorten the detection period, and to be much more stable than the colloidal gold method, which can overcome uncertainty. LP-Ag can be used to image lung tissue with COVID-19 by two-photon fluorescence microscopy (TFM).
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