Nucleic Acid (Calf Thymus-DNA, Yeast tRNA) Binding and Cytotoxic Properties of a Dinuclear (Ru,Co) Metal Polypyridyl Complex

Xi-Ling Liang; Li-Feng Tan

doi:10.1071/CH10178

RESEARCH ARTICLE

Previous Next Contents Vol 63(10)

Nucleic Acid (Calf Thymus-DNA, Yeast tRNA) Binding and Cytotoxic Properties of a Dinuclear (Ru,Co) Metal Polypyridyl Complex

Xi-Ling Liang ^A and Li-Feng Tan ^A ^B ^C

+ Author Affiliations

- Author Affiliations

^A College of Chemistry, Xiangtan University, Xiangtan 411105, PR China

^B Key Laboratory of Environment-friendly Chemistry and Application in the Ministry of Education, Xiangtan University, Xiangtan 411105, PR China.

^C Corresponding author. Email: lfwyxh@yahoo.com.cn

Australian Journal of Chemistry 63(10) 1453-1461 https://doi.org/10.1071/CH10178
Submitted: 2 May 2010 Accepted: 30 June 2010 Published: 1 October 2010

Abstract

Based on [L₂Ru{DPPZ(11–11′)DPPZ}RuL₂]⁴⁺ (where L = 1,10-phenanthroline or 2,2′-bipyridyl, DPPZ(11–11′)DPPZ = 11,11′-bi(dipyrido-[3,2-a:2′,3′-c]-phenazinyl)), a heterodinuclear (Ru,Co) metal polypyridyl complex [(phen)₂Ru{DPPZ(11–11′)DPPZ}Co(phen)₂]⁵⁺ (phen = 1,10-phenanthroline) has been designed and synthesized. A comparative study on the interaction of the complex with calf thymus DNA and yeast tRNA was investigated by UV-visible spectroscopy, fluorescence spectroscopy and viscosity measurements, as well as equilibrium dialysis and circular dichroism. The antitumour activities of the complex were evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetraazolium bromide method and Giemsa staining experiment. These results indicate that the configuration and structures of nucleic acids have significant effects on the binding behaviours of metal complexes. Furthermore, the complex shows different antitumour activities against selected tumour cell lines, and can cause cell apoptosis.

Acknowledgements

We are grateful for the support of the National Natural Science Foundation of the People’s Republic of China (21071120), the Provincial Natural Science Foundation of Hunan (09JJ6019), the Science Foundation of Hunan Provincial Education Department (08B086) and the Scientific Research Foundation of Key Lab of Environment-friendly Chemistry and Application in Ministry of Education (Xiangtan University).

References

Nucleic Acid (Calf Thymus-DNA, Yeast tRNA) Binding and Cytotoxic Properties of a Dinuclear (Ru,Co) Metal Polypyridyl Complex

Abstract

Acknowledgements

Subscriber Login