Micellization Behaviour, DNA Binding, Antimicrobial, and Cytotoxicity Studies of Surfactant–Cobalt(iii) Complexes Containing Di- and Tetramine Ligands

Rajendran Senthil Kumar; Sankaralingam Arunachalam; Vaiyapuri S. Periasamy; Christo P. Preethy; Anvarbatcha Riyasdeen; Mohammad A. Akbarsha

doi:10.1071/CH08281

RESEARCH ARTICLE

Micellization Behaviour, DNA Binding, Antimicrobial, and Cytotoxicity Studies of Surfactant–Cobalt(iii) Complexes Containing Di- and Tetramine Ligands

Rajendran Senthil Kumar ^A , Sankaralingam Arunachalam ^A ^C , Vaiyapuri S. Periasamy ^B , Christo P. Preethy ^B , Anvarbatcha Riyasdeen ^B and Mohammad A. Akbarsha ^B

+ Author Affiliations

- Author Affiliations

^A School of Chemistry, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.

^B School of Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.

^C Corresponding author. Email: arunasurf@yahoo.com

Australian Journal of Chemistry 62(2) 165-175 https://doi.org/10.1071/CH08281
Submitted: 3 July 2008 Accepted: 25 October 2008 Published: 19 February 2009

Abstract

A new class of surfactant–cobalt(iii) complexes, cis-[Co(en)₂(C₁₁H₂₃NH₂)Cl](ClO₄)₂ (1), (en = ethylenediamine) and cis-[Co(trien)(C₁₁H₂₃NH₂)Cl](ClO₄)₂ (2) (trien = triethylenetetramine) have been synthesized and characterized by UV/Vis, IR, ¹H NMR, and ¹³C NMR spectroscopic methods and elemental and metal analysis. The critical micelle concentration (CMC) values of these surfactant–cobalt(iii) complexes in aqueous solution were obtained from conductance measurements. The specific conductivity data (at 298, 308, 318, and 328 K) served for the evaluation of the temperature-dependent CMC and the thermodynamics of micellization (ΔG_m⁰, ΔH_m⁰, and ΔS_m⁰). Absorption spectroscopy, emission spectroscopy, and viscosity measurements have been used to investigate the binding of these complexes with calf thymus DNA (CT-DNA). The intrinsic binding constants (K_b) of complexes 1 and 2 were determined as 1.70 × 10⁴ M^–1 and 2.91 × 10⁴ M^–1, respectively, which suggests that complex 2 binds more strongly to CT-DNA than complex 1. These complexes were screened for their antimicrobial and cytotoxic activities against certain human pathogenic microorganisms and cervical cancer cells. The complexes showed moderate antibacterial and antifungal activities against certain selected microorganisms. The cytotoxic property of the complexes was tested on human cervical cancer cells, SiHa, adopting the 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and specific staining techniques. The complexes affected the viability of the cells significantly and the cell death was through apoptosis as seen in the changes in the nuclear morphology and cytoplasmic features.

Acknowledgements

The authors are grateful to the UGC-SAP & COSIST and DST-FIST programs. The Council of Scientific and Industrial Research (CSIR), New Delhi is gratefully acknowledged for financial support (Grant No. 01(2075)/06/EMR-II) to S.A. and a Senior Research Fellowship to R.S.K. The authors also thank UGC for sanction of a research scheme (F. 32–274/2006) to S.A.

References

Micellization Behaviour, DNA Binding, Antimicrobial, and Cytotoxicity Studies of Surfactant–Cobalt(iii) Complexes Containing Di- and Tetramine Ligands

Abstract

Acknowledgements

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