Administering Pesticide Assays in In Vivo-Implanted Biosensors

Suw Young Ly; Young Sam Jung; Chang Hyun Lee; Bang Won Lee

doi:10.1071/CH08028

RESEARCH ARTICLE

Previous Contents Vol 61(10)

Administering Pesticide Assays in In Vivo-Implanted Biosensors

Suw Young Ly ^A ^D , Young Sam Jung ^B , Chang Hyun Lee ^C and Bang Won Lee ^A

+ Author Affiliations

- Author Affiliations

^A Biosensor Research Institute in Seoul National University of Technology, 172 Gongreung 2 dong Nowon-gu, Seoul 139-743, South Korea.

^B Department of Chemistry, Korea University, Anam 5-2, Sungbook-gu, Seoul 136-701, South Korea.

^C Pyongtaek University, Division of General Education, 111 Yongyi-dong, Pyeongtaek-si, Gyeonggi-do 450-701, South Korea.

^D Corresponding author. Email: suwyoung@snut.ac.kr

Australian Journal of Chemistry 61(10) 826-832 https://doi.org/10.1071/CH08028
Submitted: 24 January 2008 Accepted: 6 August 2008 Published: 6 October 2008

Abstract

An analytical pesticide assay of O-ethyl-O-4-(nitrophenyl)phenyl phosphonothioate (EPN) was carried out using the following: a carbon nanotube paste electrode, a mercury-immobilized carbon nanotube paste electrode, a glassy carbon electrode, a metal–gold electrode, and a DNA-immobilized carbon nanotube paste electrode (DPE), which is two-fold more sensitive than other sensors. The DPE was optimized using cyclic and square wave stripping voltammetry. Linear working ranges approached 5–55 mg L^–1 EPN and the nano-range of 10–210 ng L^–1 in a 0.1 mol L^–1 NH₄H₂PO₄ electrolyte solution, with a speedy analytical time of 30-s stripping. The detection limit was 2.57 ng L^–1 (7.94 × 10^–12 mol L^–1), and the precision was 0.102% relative standard deviation (n = 15) at the 10.0 mg L^–1 EPN spike. This indicates that the method is more sensitive than common voltammetric methods. This method was applied to fruit samples using patch- and needle-type electrodes, specifically on the skin tissues of an orange and an apple. Moreover, the implanted electrode was interfaced with a fish brain cell at the electrochemical workstation. Results showed that the aforementioned method can be used to conduct a pesticide assay in neuro-treated and non-treated cell systems.

References

Administering Pesticide Assays in In Vivo-Implanted Biosensors

Abstract

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