Synthesis of NTA3-DTDA — A Chelator-Lipid that Promotes Stable Binding of His-Tagged Proteins to Membranes
Joseph G. Altin A , Martin G. Banwell B C , Phillip A. Coghlan B , Christopher J. Easton B , Michael R. Nairn B and Daniel A. Offermann BA School of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra ACT 0200, Australia.
B Research School of Chemistry, Institute of Advanced Studies, Australian National University, Canberra ACT 0200, Australia.
C Corresponding author. Email: mgb@rsc.anu.edu.au
Australian Journal of Chemistry 59(5) 302-306 https://doi.org/10.1071/CH06112
Submitted: 3 April 2006 Accepted: 26 April 2006 Published: 13 June 2006
Abstract
A six-step reaction sequence is described for the preparation of compound 1 (NTA3-DTDA), a membrane-penetrating and potent chelator that can be incorporated into liposomes and plasma membrane vesicles containing antigens and thus allowing targeted delivery of such assemblies to a variety of cells for the purposes of eliciting anti-tumour responses. Full spectroscopic characterization of this dendritic-type compound as well as certain of its precursors is reported.
Acknowledgments
We thank Lipotek Pty Ltd for financial support including the provision of a post-doctoral stipend to D.A.O.
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