
TY  - JOUR
A1  - Bradford, AM 
A1  - Bowie, JH 
A1  - Tyler, MJ 
A1  - Wallace, JC 
T1  - New Antibiotic Uperin Peptides From the Dorsal Glands of the Australian Toadlet <I>Uperoleia mjobergii</I>
JF  - Australian Journal of Chemistry
JO  - Aust. J. Chem.
Y1  - 1996
VL  - 49
IS  - 12
SP  - 1325
EP  - 1331
KW  - 
UR  - https://doi.org/10.1071/CH9961325 
N2  - The dorsal glandular extract of the toadlet Uperoleia mjobergii contains more than 20 peptides. We report the amino acid sequences of the seven major peptides: these were determined by a combination of mass spectrometry and automated Edman sequencing. Three of these peptides have 19 amino acid residues and belong to the uperin 2 group of peptides [e.g. uperin 2.6, Gly  Ile  Leu Asp Ile Ala Lys  Lys  Leu Val Gly  Gly Ile  Arg  Asn Val Leu  Gly Ile (OH)], while the other four have 17 residues and are classified as uperins 3 [e.g. Uperin 3.4, Gly Val Gly Asp Leu  Ile  Arg Lys Ala Val Ala Ala  Ile  Lys  Asn Ile Val (NH2)]. Several of these cationic peptides have been synthesized in order for bioassays to be carried out: they show significant antibiotic activity against a range of Gram-positive microorganisms. A major skin peptide from the related species Uperoleia inundata is a powerful neuropeptide named uperin 1.1 ([Ala2] uperolein ): no corresponding neuropeptide is detected in the skin glands of Uperoleia mjobergii. 
ER  -

