
TY  - JOUR
A1  - Perich, JW 
A1  - Johns, RB 
A1  - Reynolds, EC 
T1  - Synthesis of Casein-Related Peptides and Phosphopeptides. XVI. The Efficient Synthesis of the Casein-Related O-Phosphoseryl-Containing Peptide Ac-Glu-Ser(P)-Leu-Ser(P)-Ser(P)-Ser(P)-Glu-Glu-NHMe
JF  - Australian Journal of Chemistry
JO  - Aust. J. Chem.
Y1  - 1992
VL  - 45
IS  - 2
SP  - 385
EP  - 394
KW  - 
UR  - https://doi.org/10.1071/CH9920385 
N2  - The multiple-O-phosphoseryl-containing peptide, Ac-Glu-Ser (P)-Leu-Ser (P)-Ser (P)-Ser (P)-Glu-Glu-NHMe, was prepared in high yield by the use of Boc-Ser(PO3Ph2)-OH in the Boc mode of peptide synthesis for the preparation of the protected Ser(PO3Ph2)-octapeptide followed by its one-step hydrogenolytic deprotection with platinum oxide. Peptide synthesis was performed by the repetitive excess mixed-anhydride coupling procedure and 40% CF3CO2H/CH2Cl2 for removal of the Boc group from intermediate peptides. The phosphopeptide was found to be greater than 99% pure by C8 r.p.-h.p.1.c. analysis, and was readily characterized by amino acid analysis, 13C n.m.r. spectroscopy and f.a.b. mass spectrometry. 
ER  -

