
TY  - JOUR
A1  - Martin, RF 
T1  - The chemistry of the DNA-p-Nitrophenylhydrazine reaction
JF  - Australian Journal of Chemistry
JO  - Aust. J. Chem.
Y1  - 1972
VL  - 25
IS  - 2
SP  - 401
EP  - 406
KW  - 
UR  - https://doi.org/10.1071/CH9720401 
N2  - The chemistry of the reaction between DNA and pNPH has been investigated by isolation and analysis of the chromogen. The perchlorate of the cationic chromo-gen was prepared from the reineckate by anion exchange chromatography, and samples from both DNA-pNPH and furfuryl alcohol-pNPH reaction mixtures were found to have identical i.r. and p.m.r. spectra. The data obtained from the p.m.r. spectra were consistent with a reaction scheme involving formation of a pyridazinium cation which subsequently yields the chromophore on treatment with alkali. 
ER  -

