
%0 Journal Article
%A Guzzo-Pernell, Nancy 
%A Lawlor, John M. 
%A Tregear, Geoffrey W. 
%A Haralambidis, Jim 
%T Triplex Formation by an Oligonucleotide Containing the Novel Modified Nucleoside 2&#8242;-Deoxy-<emph type="2">N</emph><emph type="7">4</emph>-phenylcarbamoylcytidine
%J Australian Journal of Chemistry
%D 1998/11/17
%V 51
%N 11
%P 965-972
%K 
%R https://doi.org/10.1071/C98062
%U https://www.publish.csiro.au/paper/C98062 
%X 2&#8242;-Deoxy-N4-phenylcarbamoylcytidine (Pc) was designed for triplex recognition of the cytosine&#123;guanine base pair at physiological pH values to assist in the development of triplex-forming oligomers. Thermal denaturation of a triplex mixture in which the third strand incorporated Pc as a central nucleoside showed that, at pH 6&#183;1, Pc binds selectively&#8212;though weakly&#8212;to the target cytosine&#8212;guanine and also to guanine&#8212;cytosine; but the gel mobilities of the same mixtures indicate the possibility of specificity for the target cytosine-guanine base pair at pH values higher than 6&#183;1. The result may provide leads useful in the development of improved triplex-forming oligomers. 
