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Ngaio Smith obtained her BSc (Hons) and PhD from the University of Sydney under the supervision of Professor Jacqui Matthews. Her work focusses on the interactions of transcription factors and DNA, particularly in the LIM-HD family of proteins. A key area of interest of her work has been how protein–protein interactions affect DNA-binding specificity and gene regulation.

Jacqui Matthews is a graduate of UNSW and was awarded her PhD from the University of Cambridge, working with Sir Alan Fersht to define the principles underlying protein folding. She undertook postdoctoral work with Professor Richard Simpson at the Ludwig Institute for Cancer Research in Melbourne, where she first met and worked with Ed Nice. She developed an independent research program at The University of Sydney centered on biomolecular interactions, particularly the assembly of multiprotein transcriptional complexes involved in cell-specific transcriptional regulation.