¹H-N.M.R. and Theoretical Studies of the Conformation of the Antibiotic Lincomycin

Abstract

One- and two-dimensional ¹H n.m.r. spectra of the amino glycoside antibiotic lincomycin have been measured and analysed. One-dimensional n.m.r. techniques applied included selective decoupling, resolution enhancement, and n.O.e. difference experiments. Two-dimensional n.m.r. techniques included chemical shift correlated spectroscopy (COSY) and n.O.e. spectroscopy (NOESY) experiments.

A theoretical conformational analysis of the side chain of the sugar moiety has also been done by using a simplified potential energy program. Together, the theoretical and experimental (coupling constant and n.O.e.) data have allowed the conformation of lincomycin to be determined.