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RESEARCH ARTICLE
Contents Vol 75(2)

Fluorescent Reporters for Antimicrobial Peptides

Yuning Hong https://orcid.org/0000-0002-8085-1651 A C and Wenyi Li https://orcid.org/0000-0003-3584-0301 B C
+ Author Affiliations
- Author Affiliations

A Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Vic. 3086, Australia.

B Bio21 Institute, Melbourne Dental School, University of Melbourne, Melbourne, Vic. 3010, Australia.

C Corresponding authors. Email: y.hong@latrobe.edu.au; wenyi.li@unimelb.edu.au




Dr Wenyi Li obtained his doctoral degree from The University of Melbourne in 2016, working with Professors John Wade and Frances Separovic. His thesis on antimicrobial peptide development won the Graham Johnston Best Thesis Award from the RACI and the University of Melbourne’s Monica Reum Memorial Prize. He then worked with Professor Christian Hackenberger at the Leibniz Institute of Molecular Pharmacology, Germany, as a Leibniz-DAAD post-doctoral fellow conducting research in protein semisynthesis and chemical ligation. In 2018, he returned to The University of Melbourne to work on antibacterial polymers and peptides in the groups of Professors Neil O’Brien-Simpson and Greg Qiao.

Australian Journal of Chemistry 75(2) 2-8 https://doi.org/10.1071/CH21070
Submitted: 15 March 2021  Accepted: 31 May 2021   Published: 16 June 2021

Abstract

Antimicrobial peptides (AMPs), a part of the natural defence against pathogens, have been considered as alternative antibiotics to combat the increase of antimicrobial resistance (AMR). Given the advanced development of fluorescent probes, extensive research has been focussed on understanding the physiological processes of the interaction between AMPs and bacteria. To better guide the choice of suitable fluorescent reporters for the mechanism study of AMPs, in this review, we summarise a toolbox of commonly used fluorescent reporters for AMP studies, including intrinsic fluorescent reporters, conventional fluorophores, and recently developed aggregation-induced emission (AIE) fluorogens.

Keywords: antimcirobial peptide, peptide labelling, fluorescent probes, mode of action, intrinsic fluorescence, conventional fluorophores, aggregation-induced emission fluorogens, antimicrobial resistance.


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