Preparation of Protein–Polymer Conjugates: Copolymerisation by RAFT
Fei Huang A , Judith A. Scoble B , John Chiefari A and Charlotte C. Williams
B C
+ Author Affiliations
- Author Affiliations
A CSIRO Manufacturing, Bag 10, Bayview Avenue, Clayton, Vic. 3168, Australia.
B CSIRO Manufacturing, 343 Royal Parade, Parkville, Vic. 3052, Australia.
C Corresponding author. Email: Charlotte.Williams@csiro.au
Australian Journal of Chemistry 73(10) 1027-1033 https://doi.org/10.1071/CH19514
Submitted: 13 October 2019 Accepted: 28 February 2020 Published: 2 June 2020
Journal Compilation © CSIRO 2020 Open Access CC BY-NC-ND
Abstract
A method for the preparation of protein–polymer conjugates is presented that represents a different approach to current conjugation techniques. A protein–polymer conjugate was prepared by reversible addition–fragmentation chain-transfer (RAFT) copolymerisation, where one of the species of monomer used contains a protein. The enzyme horseradish peroxidase (HRP) was functionalised with an acrylate group via a polyethylene glycol (PEG) linker to the protein surface lysine residues. The PEG linker promoted aqueous solubility of the acrylate group, which led to an improved yield of HRP functionalisation. RAFT copolymerisation with N-acryloylmorpholine (NAM) resulted in synthesis of an HRP-RAFT copolymer, with full retention of the enzyme’s activity.
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