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RESEARCH FRONT
Contents Vol 67(12)

RNA and RNA–Protein Complex Crystallography and its Challenges

Janine K. Flores A B , James L. Walshe A B and Sandro F. Ataide A C
+ Author Affiliations
- Author Affiliations

A School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia.

B These authors contributed equally to this paper.

C Corresponding author. Email: sandro.ataide@sydney.edu.au




Janine Flores received her B.Sc. in biochemistry and psychology from the University of Sydney in 2012. She completed her B.Sc. (Honours) in biochemistry in 2013 in the Ataide laboratory, where her research focused on determining the crystal structure of the protein and RNA components of the eukaryotic signal recognition particle (SRP). Currently, Janine is a Ph.D. candidate at the Ataide laboratory focusing on the structural characterisation of ribonucleoprotein complexes.


James Walshe received his B.Sc. degree from the University of Western Australia (UWA) in 2010. He completed his B.Sc. (Honours) in biochemistry in 2011, working in Alice Vrielink's laboratory (UWA) on metal binding sites found in the crystal structure of N. meningitidis Lipopolysaccharide transferase A. He is currently a Ph.D. candidate at the Ataide laboratory (University of Sydney) investigating the structural interactions of ribonucleoproteins that associate with non-coding RNAs.


Dr Sandro Ataide received his B.Sc. in chemistry in 1999 from UNICAMP (Brazil) and obtained his M.Sc. in biochemistry from the University of Sao Paulo (Brazil) in 2001. He was awarded his Ph.D. by The Ohio State University (USA) in 2006. Aiming to gain better experience in the RNA and crystallography field, he conducted research on the complex signal recognition particle (SRP) and ribosome while working as a post-doctorate researcher at Jennifer Doudna's laboratory at UC Berkeley (USA) and Dr Nenad Ban's laboratory at ETH Zurich (Switzerland). He joined the University of Sydney as a lecturer in 2012 to continue his work on structural and molecular characterisation of RNA and RNA-protein complexes.

Australian Journal of Chemistry 67(12) 1741-1750 https://doi.org/10.1071/CH14319
Submitted: 20 May 2014  Accepted: 24 June 2014   Published: 19 August 2014

Abstract

RNA biology has changed completely in the past decade with the discovery of non-coding RNAs. Unfortunately, obtaining mechanistic information about these RNAs alone or in cellular complexes with proteins has been a major problem. X-ray crystallography of RNA and RNA–protein complexes has suffered from the major problems encountered in preparing and purifying them in large quantity. Here, we review the available techniques and methods in vitro and in vivo used to prepare and purify RNA and RNA–protein complex for crystallographic studies. We also discuss the future directions necessary to explore the vast number of RNA species waiting for their atomic-resolution structure to be determined.


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