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RESEARCH FRONT
Contents Vol 66(7)

Chemical Cross-linking and Mass Spectrometry for the Structural Analysis of Protein Assemblies

Antonio N. Calabrese A and Tara L. Pukala A B
+ Author Affiliations
- Author Affiliations

A School of Chemistry and Physics, The University of Adelaide, Adelaide, SA 5005, Australia.

B Corresponding author. Email: tara.pukala@adelaide.edu.au




Antonio Calabrese is a Ph.D. candidate at the University of Adelaide under the supervision of both Dr Tara Pukala and Professor John H. Bowie. His research interests include the development of novel chemical cross-linking mass spectrometry methodologies and the use of complementary techniques to probe the structures of protein assemblies of biological importance.


Dr Tara Pukala obtained a Ph.D. from the University of Adelaide (with J. H. Bowie), which was followed by a postdoctoral role at the University of Cambridge, UK (with C. V. Robinson). She returned to a lectureship position at the University of Adelaide in 2008, where she has established a research group focussed on the development and application of mass spectrometric and related technologies for the investigation of biomolecular systems.

Australian Journal of Chemistry 66(7) 749-759 https://doi.org/10.1071/CH13164
Submitted: 8 April 2013  Accepted: 23 May 2013   Published: 1 July 2013

Abstract

Cellular functions are performed and regulated at a molecular level by the coordinated action of intricate protein assemblies, and hence the study of protein folding, structure, and interactions is vital to the appreciation and understanding of complex biological problems. In the past decade, continued development of chemical cross-linking methodologies combined with mass spectrometry has seen this approach develop to enable detailed structural information to be elucidated for protein assemblies often intractable by traditional structural biology methods. In this review article, we describe recent advances in reagent design, cross-linking protocols, mass spectrometric analysis, and incorporation of cross-linking constraints into structural models, which are contributing to overcoming the intrinsic challenges of the cross-linking method. We also highlight pioneering applications of chemical cross-linking mass spectrometry approaches to the study of structure and function of protein assemblies.


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