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Contents Vol 64(5)

Raman Spectroscopic Imaging of Cholesterol and Docosahexaenoic Acid Distribution in the Retinal Rod Outer Segment

Zachary D. Schultz
+ Author Affiliations
- Author Affiliations

Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46566, USA. Email: Schultz.41@nd.edu

Australian Journal of Chemistry 64(5) 611-616 https://doi.org/10.1071/CH11019
Submitted: 10 January 2011  Accepted: 23 March 2011   Published: 30 May 2011

Abstract

Raman vibrational spectroscopic imaging was performed on retinal rod cells isolated from bullfrogs (Rana catesbeiana). The Raman spectra enable determination of the lipid and protein rich rod outer segment (ROS) from the nucleus and inner segment of the cell. Peak fitting analysis of spectra obtained from individual rod photoreceptor cells show characteristic vibrational modes that can be associated with cholesterol and docosahexaenoic acid-containing lipids. These results provide direct observations of biomolecular gradients in the rod photoreceptor cells, which, thus far, have been based on indirect detergent extracts and histochemical analysis with indicators such as filipin. The detected biomolecules are associated with regulation of the integral membrane protein rhodopsin, and methods capable of direct observation of these biomolecules offer new routes to exploring their role in the regulation of cellular processes.


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