Production of Omega-3 Triacylglycerol Concentrates using a New Food Grade Immobilized Candida antarctica Lipase B

Jaroslav A. Kralovec; Weijie Wang; Colin J. Barrow

doi:10.1071/CH10087

RESEARCH FRONT

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Production of Omega-3 Triacylglycerol Concentrates using a New Food Grade Immobilized Candida antarctica Lipase B

Jaroslav A. Kralovec ^A , Weijie Wang ^A and Colin J. Barrow ^B ^C

+ Author Affiliations

- Author Affiliations

^A Ocean Nutrition Canada Ltd, 101 Research Drive, Dartmouth, NS, B2Y 4T6, Canada.

^B School of Life and Environmental Sciences, Deakin University, Geelong, Vic. 3217, Australia.

^C Corresponding author. Email: cbarrow@deakin.edu.au

Australian Journal of Chemistry 63(6) 922-928 https://doi.org/10.1071/CH10087
Submitted: 16 February 2010 Accepted: 29 March 2010 Published: 11 June 2010

Abstract

Triacylglycerol concentrates of eicosapentaenoic and docosahexaenoic omega-3 fatty acids were synthesized either via transesterification or esterification of glycerol with the corresponding ethyl ester or free fatty acid concentrates, respectively. A newly developed food grade immobilized Candida antarctica lipase B system using an Amberlite FPX-66 hydrophobic matrix, was compared with a commercially available non-food grade commercial system, for their ability to catalyze these reactions. For either system, the transesterification required higher temperature (90°C) than esterification (70°C) to achieve maximum triacylglycerol yields. The newly developed immobilized system efficiently catalyzes the esterification of free fatty acids with glycerol and differs from the existing commercial system in that it is food grade and has a more uniform and larger particle distribution. The new system significantly improves flow in a packed bed reactor, enabling multiple reuse of the catalyst for up to 80 repeats.

Acknowledgements

We are grateful to the Industrial Research Program of National Research Council of Canada for the financial support (Project # 674301). We thank Dr Erick Reyes-Suarez for running the NMR spectra, Ms. Ray Townsley for excellent technical assistance and, and Mr. H. C. Holm from Novozymes for numerous advice and stimulating discussions.

References

Production of Omega-3 Triacylglycerol Concentrates using a New Food Grade Immobilized Candida antarctica Lipase B

Abstract

Acknowledgements

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