Processing Windows for Forming Silk Fibroin Biomaterials into a 3D Porous Matrix

Hyeon Joo Kim; Hyun Suk Kim; Akira Matsumoto; In-Joo Chin; Hyoung-Joon Jin; David L. Kaplan

doi:10.1071/CH05170

RESEARCH FRONT

Processing Windows for Forming Silk Fibroin Biomaterials into a 3D Porous Matrix

Hyeon Joo Kim ^A , Hyun Suk Kim ^A ^B , Akira Matsumoto ^A , In-Joo Chin ^B , Hyoung-Joon Jin ^B and David L. Kaplan ^A ^C

+ Author Affiliations

- Author Affiliations

^A Department of Biomedical Engineering, Bioengineering & Biotechnology Center, Tufts University, Medford, MA 02155, USA.

^B Department of Polymer Science and Engineering, Inha University, Incheon 402-751, Korea.

^C Corresponding author. Email: david.kaplan@tufts.edu

Australian Journal of Chemistry 58(10) 716-720 https://doi.org/10.1071/CH05170
Submitted: 8 September 2005 Accepted: 23 October 2005 Published: 8 November 2005

Abstract

In the present study we clarify phase diagrams related to silk fibroin processing into three-dimensional porous structures useful for biomaterials and for scaffolds in tissue engineering. All-aqueous and organic solvent (hexafluoroisopropanol) modes of processing are compared relative to solution concentration of silk protein polymer and size of porogen (NaCl particles). The results clarify the range of conditions under which these biomaterial matrices can be formed, with a broader range of pore sizes and smoother surface morphology generated from the organic solvent process. These structures are directly applicable to fundamental studies of protein-based biomaterial assembly as well as cell interactions and tissue formation with these systems.

Acknowledgements

We thank the NIH (ED002520 and EB003210) and the NSF (DMR) for support of this work.

References

Processing Windows for Forming Silk Fibroin Biomaterials into a 3D Porous Matrix

Abstract

Acknowledgements

Subscriber Login