Electrochemical Detection of Yeast Responses to Catabolizable Molecules
Keith H. R. Baronian A B , Saroja Gurazada A and Ancy Thomas AA School of Applied Science, Christchurch Polytechnic Institute of Technology, Christchurch, New Zealand.
B Corresponding author. Email: baroniank@cpit.ac.nz
Australian Journal of Chemistry 58(4) 270-274 https://doi.org/10.1071/CH04258
Submitted: 29 October 2004 Accepted: 1 February 2005 Published: 5 April 2005
Abstract
Cells harvest energy from energy-rich molecules by oxidation of the molecule and the subsequent transfer of electrons to catabolic redox compounds. This transfer can be intercepted by artificial redox compounds (mediators), and the reduction of the mediator can be easily monitored by several electrochemical techniques. This work describes the use of steady-state voltammetry to monitor the catabolic responses of two yeast species to specific molecules.
Acknowledgments
Arxula adeninivorans was kindly made available by Prof. Dr. Gotthard Kunze, Institut für Pflanzengenetik und Kulturflanzenforschung, 06446 Gatersleben, Germany. hER Saccaromyces cerevisiae was kindly made available by Barbara Thompson, Environmental Science and Research (ESR), Christchurch, New Zealand. This work was funded in part by the Foundation for Research, Science and Technology, New Zealand.
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