
TY  - JOUR
A1  - Martinez, Pastur GJ 
A1  - Arena, ME 
T1  - <I>In vitro</I> Propagation of <I>Nothofagus obliqua</I> (Fagaceae).
JF  - Australian Journal of Botany
JO  - Aust. J. Bot.
Y1  - 1995
VL  - 43
IS  - 6
SP  - 601
EP  - 607
KW  - 
UR  - https://doi.org/10.1071/BT9950601 
N2  - Suitable culture conditions were investigated for in vitro propagation through shoot culture of seedlings and the acclimatization of the rooted plantlets of Nothofagus obliqua. Explant size and leaf expansion were critical for culture initiation. A medium with a low salt concentration and the addition of L-glutamine, as broadleaved tree medium, was suitable for shoot multiplication and it was influenced by both cytokinin and auxin. A multiplication rate of x5 has been obtained with 0.55 micro M benzyladenine (BA), and 0.09 micro M BA and 0.09 micro M IBA at day 63. Rooted shoots (51.9%) were obtained with 0.61 IBA and 1 week darkness at the induction stage. Rooted plants were then successfully acclimatised and 75% survival rate was obtained after 4 months. 
ER  -

